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Title[redir]:
Transcript assembly and abundance estimation from RNA-Seq reveals thousands of new transcripts and switching among isoforms - PMC
Description:
High-throughput mRNA sequencing (RNA-Seq) holds the promise of simultaneous transcript discovery and abundance estimation1-3. We introduce an algorithm for transcript assembly coupled with a statistical model for RNA-Seq experiments that produces ...
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Keywords {🔍}
doi, isoforms, transcripts, transcript, cufflinks, expression, fragments, genes, reads, time, abundance, rnaseq, abundances, fragment, supplementary, google, scholar, pubmed, pmc, genome, isoform, article, assembly, free, sequencing, relative, alignments, catalog, gene, model, estimates, data, methods, set, differential, rna, nature, estimation, regulation, fig, cell, splicing, graph, total, transcriptional, mrna, multiple, tophat, cdna, differentiation,
Topics {✒️}
paired-end 75bp rna-seq paired-end rna-seq data nih grants r01-lm006845 conducted chip-seq experiments a-half-lim domains 3 dna-free total rna paired-end rna-seq rna-seq reveals thousands massive-scale rna sequencing vivo protein-dna interactions pmc beta search endpoint rt-pcr validation form-specific rt-pcr information-theoretic metric derived massive-scale mrna sequencing silico rna-seq sample rna-seq fragment densities turbo dna-free high-identity est alignments qiaex ii kit post-transcriptionally induced expression post-transcriptional regulatory effects multi-promoter genes featured rna polymerase ii skeletal muscle determination high-throughput sequencing chip-seq signal [google scholar] 19 minimum-cost maximum matching post-transcriptional isoform switches differential post-transcriptional processing transcript-level abundance estimates muscle-specific functions rna-seq experiments illumina flow cell sequence alignment/map format major primary transcript paired-end case paired end adapters skeletal muscle development rna-seq data mrna-seq experiments rna-seq studies paired-end primers genomic structural variations6 disjoint genomic loci distinct expression dynamics qiaquick pcr column integrated dna technologies ultraconserved dna elements
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