NCBI . NLM . NIH . GOV {
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Title[redir]:
Sequence- and structure-specific RNA processing by a CRISPR endonuclease - PMC
Description:
Many prokaryotes contain genomic clustered regularly interspaced short palindromic repeats (CRISPRs) that confer resistance to invasive genetic elements. Central to this immune system is the production of CRISPR-derived RNAs (crRNAs) following ...
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Keywords {🔍}
doi, csy, rna, fig, pubmed, google, scholar, crispr, pmc, precrrna, nucleotide, cleavage, sequence, article, repeat, science, cas, data, crrna, free, phosphate, processing, recognition, substrate, activity, base, complex, interactions, groove, stemloop, residues, nlm, major, shown, hairpin, arg, crystal, structure, vitro, stem, helix, pair, ncbi, search, site, rnas, mechanism, sequencespecific, sequences, unique,
Topics {✒️}
n-peptide/boxb rna interaction sequence-specific pre-crrna processing full-length crrna repeat metal ion-independent reaction x-ray data collection acid phenol-chloroform extracted rna stem-loop straddles pmc beta search supplementary materials included crispr repeat stem-loop permit sequence-specific interactions cognate pre-crrna substrate vitro transcribed pre-crrna repeat-derived stem-loop β6-β7 hairpin turn predicted stem-loop structure pseudomonas aeruginosa ucbpp-pa14 structure-specific rna processing n-terminal ferredoxin fold protein/rna interactions required rna-binding conformation superposes csy4-rna interactions required arginine-rich helix α3 closing base pair crispr-processing endonucleases case advanced light source repeat stem-loop penultimate base pair stem-loop structure n-terminal ferredoxin pre-crrna processing contained wild-type csy4 predicted stem-loop short rnas participates pre-crrna substrate short rna oligonucleotides pre-crrna transcripts cleave pre-crrna pre-crrna recognition final edited form exogenous metal ions strands β6-β7 structure-specific processing sequence-specific interaction csy4-rna complex structure crispr repeat sequence identical repeats interspersed acid phenolchloroform extracted strongly conserved tyrosine structure-specific interactions
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