
NCBI . NLM . NIH . GOV {
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Title[redir]:
Sam68 marks the transcriptionally active stages of spermatogenesis and modulates alternative splicing in male germ cells - PMC
Description:
Sam68 plays an essential role in mouse spermatogenesis and male fertility. Herein, we report an interaction between Sam68 and the phosphorylated forms of the RNA polymerase II (RNAPII) in meiotic spermatocytes. RNase treatment decreased but did not ...
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Keywords {🔍}
sam, doi, cells, germ, splicing, rnapii, spermatocytes, pubmed, google, scholar, rna, figure, cell, expression, meiotic, pachytene, nuclear, exon, mouse, protein, pmc, phosphorylated, spermatogenesis, binding, antibodies, male, article, alternative, sgce, free, gene, mice, transcription, wildtype, knockout, stage, proteins, required, min, staining, analysis, testis, serine, results, extracts, buffer, rnase, chromatin, prophase, supplementary,
Topics {✒️}
5 mm isopropyl-β-d-thiogalactopyranoside cy3-conjugated anti-mouse iggs fitc-conjugated anti-rabbit iggs proteina/agarose/salmon sperm dna proteina-sepharose beads pre-adsorbed serine/arginine-rich proteins 9g8 x-linked lymphocyte-regulated genes m-mlv reverse transcriptase long-term fellowship grant cyclin d1 proto-oncogene nuclear matrix-attached insoluble nuclear kinase sik/brk tissue-specific splicing variants c57/b6 wild-type pmc beta search protein a-sepharose beads radio-labelled wild-type rna-binding protein implicated cross-linked germ cells gonadotropin-dependent follicle development high-salt wash buffer streptavidine beads pre-adsorbed phosphorylated carboxyl-terminal domain adult wild-type mice rna binding-independent fashion anti-p-ser2 rnapii 1093/nar/gkr085 sam68 marks post-meiotic germ cells age-related bone loss direct protein–protein interaction testis-specific histone expressed sgce pre-mrna showed spo11−/−spermatocytes display features purified gst-fusion proteins testis-specific splicing regulator pre-initiation transcriptional complex 200 ng/μl yeast trna generating atm−/−spo11+/− mice sam68 carboxyl-terminal region testis-specific gene expression post-transcriptional gene regulation mid-pachytene spermatocytes starting rna-binding protein sam68 sam68 rna-binding protein wild-type pachytene spermatocytes testis-specific splicing regulators mouse male germline proteina-sepharose beads modulates alternative splicing grp33/sam68/gld-1 homology
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