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Title[redir]:
Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition - PMC
Description:
We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome ...
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Keywords {🔍}
dna, libraries, library, pcr, sequencing, genome, coverage, adaptor, doi, methods, human, size, fragmentation, genomic, nextera, sequence, standard, coli, min, reaction, transposase, insertion, figure, reads, construction, method, illumina, preparation, file, bias, pmc, additional, barcode, pubmed, content, data, transposasecatalyzed, distribution, buffer, input, google, scholar, number, read, article, protocols, sample, samples, carried, free,
Topics {✒️}
long-insert mate-paired libraries cell-line-discordant snp calls time-dependent endonuclease-based fragmentation pre-sequencing real-time pcr primer-embedded sample-specific barcode strand-specific transcriptome sequencing pmc beta search wild-type tn5 transposase streptavidin-coupled magnetic beads transposase-catalyzed adaptor insertion gel-based size-selection gel-based size selection health/national heart lung long illumina-compatible adaptor uniquely occurring read-pairs transposase-mediated adaptor insertion bar-coded multiplexed sequencing sequencing-ready shotgun library sequence alignment/map format mapping-independent insert size primer-embedded barcode sequence append platform-specific primers tn5/is50 target recognition post-pcr sample clean cyan/light green triangles labor-intensive enzymatic manipulations read-pairs sampled versus rapidly preparing sequencing-ready fragmentase-treated phage samples sequencing-compatible primer sites supplementary materials included efficiently construct high-complexity real-time dna sequencing transposase-based libraries subjected sequence-ready barcoded libraries cell-line dna derived real-time pcr thermocycler mid blue/brown/orange pcr-free version nuclease-free water post-sequencing deconvolution paired-end 76-cycle run transposase-based library nextera primer cocktail pcr-free protocol post-pcr library massively parallel sequencing amplification-free versions significantly longer fragments positional weight matrix
Questions {❓}
- What would you do if you could sequence everything?
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