
NCBI . NLM . NIH . GOV {
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Title[redir]:
Translation initiation on mammalian mRNAs with structured 5′-UTRs requires DExH-box protein DHX29 - PMC
Description:
Eukaryotic protein synthesis begins with assembly of 48S initiation complexes at the initiation codon of mRNA, which requires at least 7 initiation factors (eIFs). First, 43S preinitiation complexes comprising 40S ribosomal subunits, eIFs 3, 2, 1, ...
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Keywords {🔍}
dhx, complexes, figure, mrna, doi, initiation, complex, ribosomal, formation, subunits, mrnas, rna, lanes, google, scholar, pubmed, scanning, pestova, eif, codon, assembled, ires, translation, eifa, iress, pmc, subunit, stem, lane, utrs, activity, helicase, aug, binding, mrnabinding, unwinding, free, article, caagus, eifs, toeprints, panel, cleft, conformational, presence, protein, rrl, proteins, hellen, eukaryotic,
Topics {✒️}
promote eif4f/4a/4b-independent attachment eif4f/4a/4b acted synergistically recombinant eifs 1/1a/4a/4b/5/5b promote eif4a/4b/4f-independent attachment eif2/gtp/met-trnameti ternary complexes eif2/3/1/1a/4a/4b/4f eif4f/4a/4b cooperatively unwind compared chemical/enzymatic foot-printing monitor [35s]met-trnameti incorporation sdg-purified 43s/dxh29 complexes experimental procedures plasmids de la cruz eif4a/4g/4b assist scanning pmc beta search splicing factor prp2/dhx16 cell-free translation extracts putative dexh-box helicase eif4f/4a/4b bind reject potential mis-matches representative dexh-box proteins dead/dexh-box proteins dead-box protein ded1p combination simulates mrna-attached total aminoacyl-trna synthetases c-terminally located helicase eif4a/4g/4b act dexh-box protein dhx29 dead-box rna helicase 40s/ires complex formation dhx29-bound 40s-ribosomal complexes codon-anticodon base-pairing codon–anticodon base-pairing p-site ccu codon foot-printing experiments revealed pre-assembled 48s complexes c-terminally truncated δdhx29 eif4a/4g/4b bind mediate end-independent initiation atp-free states thin-layer chromatography analysis free 43s complexes native β-globin mrna 7 μm [γ-32p]atp cell-free extracts preassembled ires/40s complexes cap-proximal mrna segment 7 μm [α-32p]atp additional rna-binding domains cold spring harbor caa-gus stem2-4 mrnas
Questions {❓}
- But what is the role of different factors in ribosomal movement per se?
- How does DHX29 stimulate 48S complex formation on mRNAs with structured 5′-UTRs?
- What determines whether mammalian ribosomes resume scanning after translation of a short upstream open reading frame?
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- Monthly income for https://www.nlm.nih.gov/accessibility.html
- What's the financial outcome of https://www.nlm.nih.gov/careers/careers.html?
- Financial intake of https://www.nlm.nih.gov/
- How much profit does https://www.nih.gov/ make?
- What's the income of https://www.hhs.gov/?
- Profit of https://www.usa.gov/
Analytics and Tracking {📊}
- Google Analytics
- Google Analytics 4
- Google Tag Manager
Libraries {📚}
- jQuery
- jQuery module (jquery-3.6.0)
- Zoom.js
Emails and Hosting {✉️}
Mail Servers:
- nihcesxway.hub.nih.gov
- nihcesxway2.hub.nih.gov
- nihcesxway3.hub.nih.gov
- nihcesxway4.hub.nih.gov
- nihcesxway5.hub.nih.gov
Name Servers:
- dns1-ncbi.ncbi.nlm.nih.gov
- dns2-ncbi.ncbi.nlm.nih.gov
- lhcns1.nlm.nih.gov
- lhcns2.nlm.nih.gov
CDN Services {📦}
- Ncbi