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Keywords {🔍}

autophagy, doi, genes, cells, figure, screen, cell, sirna, mtorc, levels, pubmed, google, scholar, regulation, knockdown, kinase, lcgfp, cytokines, pmc, analysis, pathways, type, signaling, article, clcf, iii, gene, free, conditions, data, table, ngml, human, fyvedsred, function, significant, growth, stat, presence, treatment, normal, quantification, hits, regulate, assay, hit, mtor, cellular, led, transfected,

Topics {✒️}

cis-regulatory elements/transcription factor lc3-gfp psrp-beclin1 knock image-based high-throughput screen genome-wide interactome screens total lc3-gfp intensity/cell serum-free medium supplemented tailed student t-test 2-tailed student t-test high-throughput fluorescent microscope pmc beta search lc3-gfp positive autophagosomes/cell genome-scale rnai screens fyve-dsred vesicle recruitment combining multi-level screening additional high-throughput assays pi3 kinase/akt signaling secondary lc3-gfp assay lc3-gfp secondary assay h4 lc3-gfp cells lc3 ii/tubulin ratio h4 fyve-dsred cells atp-competitive mammalian target high-throughput characterization assays serum-free medium motif-based gene sets ptdins3p reporter fyve-dsred primary screen z-scores irdye-800-conjugated secondary antibody methods cell lines serum-free media consistently significant z-scores lc3-gfp positive autophagosomes tf-binding sites defined human srf-related proteins primary screen z-score cell-based screening method class iii pi amino acid-dependent regulation negatively regulating autophagy supplementary materials included lc3-gfp reporter rabbit-anti-rps6 phospho-235/236 multiple cell lines references byfield mp promoting intracellular recycling h4 lc3-gfp joshi-tope high-throughput characterization nih imagej64 software vesicular fyve-dsred

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