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Title[redir]:
Regulatory T-cell suppressor program co-opts transcription factor IRF4 to control TH2 responses - PMC
Description:
In the course of infection or autoimmunity, particular transcription factors orchestrate the differentiation of TH1, TH2 or TH17 effector cells, the responses of which are limited by a distinct lineage of suppressive regulatory T cells (Treg). Treg ...
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Keywords {π}
cells, treg, mice, irf, foxp, doi, cell, expression, foxpcre, irffl, fig, irfflflfoxpcre, pubmed, google, scholar, data, regulatory, control, genes, nature, supplementary, irfdeficient, effector, shown, increased, analysis, pmc, differentiation, factor, article, production, showed, serum, results, qpcr, subset, independent, igg, gene, suppressor, transcription, cytokine, plasma, flow, transcriptional, gfp, littermates, foxpdtr, open, performed,
Topics {βοΈ}
carboxy-terminal 65-amino-acid sequence diphtheria-toxin-treated foxp3dtr mice c-terminal 65-amino-acid sequence mononuclear-cell-dominated pathology typical anti-foxp3-coated magnetic beads signalling-dependent post-translational modifications including ifn-Ξ³-dependent igg2a specific pathogen-free conditions thy1-specific macs beads cd4-specific macs beads underwent cre-mediated recombination biotinylated anti-ige antibody th2-specific gene set foxp3-dependent developmental program transcription factor lsirf/irf4 cd3-stimulated splenic irf4fl/flfoxp3cre cre-mediated recombination results irf4-deficient treg subset pmc beta search enzyme-linked immunosorbent assay irf4-bound chromatin isolated diphtheria toxin treatment healthy irf4fl/β foxp3cre/wt ige class-switch recombination irf4-dependent manner suggested irf4fl/flfoxp3cre mice showed treg-deficient foxp3β mice irf4fl/β foxp3cre/wt mice irf4fl/flfoxp3cre mice compared treg-mediated th2 suppression diseased irf4fl/flfoxp3cre mice irf4-deficient treg cells regulatory t-cell differentiation wild-type treg cells irf4fl/flfoxp3cre littermates suppress increased ifn-Ξ³ production οΏ½treg-specificβ genes decreased regulatory t-cell function icos-deficient treg cells ige-producing plasma cells unlike irf4fl/flfoxp3cre mice irf4-sufficient treg cells th1 cytokine ifn-Ξ³ supplementary materials included treg cells resulted irf4-sufficient foxp3β cd4+ full methods wild-type b6 splenocytes green fluorescent protein foxp3-specific antibody
Questions {β}
- Foxp3 and natural regulatory T cells: key to a cell lineage?
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