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Keywords {🔍}

cells, twist, cell, cancer, breast, stem, expression, doi, mcftwist, subpopulation, pubmed, google, scholar, cdcdlow, lines, figure, mcf, phenotype, mcfatwist, pmc, increased, aldh, flow, tumors, article, twistoverexpressing, data, promoter, row, res, results, free, shown, mcfa, parental, levels, compared, efflux, analyzed, cytometry, cdcd, rhodamine, tumor, observed, mdamb, knockdown, transient, ability, markers, overexpression,

Topics {✒️}

twist+/cd44+/cd24-/low subpopulation required stable twist-overexpressing mcf-10a/twist twist-overexpressing cd44+/cd24-/low subpopulation established cd44+/cd24-/low/aldh+ phenotype twist+/cd44+/cd24-/low subpopulation compared mcf-10a/twist cell line sonic hedgehog-patched-gli pathway cd44+/cd24-/low subpopulation stabilized purified cd44+/cd24-/low subpopulation high cd44+/cd24-/low subpopulation twist+/cd44+/cd24-/low subpopulation twist+/cd44+/cd24+ purified subpopulation cd44+/cd24-/low subpopulation compared twist knockdown mcf-10a/twist higher cd44+/cd24-/low subpopulation high cd44+/cd24-/low levels independent mcf-10a/twist clones analyzed multiple mcf-10a/twist twist-overexpressing cell lines mcf-7/twist cell line sonic hedgehog/patch pathway ultra low-attachment plate mcf-7/twist cell lines cd44+/cd24-/low subpopulation purified cd44+/cd24-/low mda-mb-231 cell lines twist-expressing mcf-7 cells cd44+/cd24-/low phenotype twist-overexpressing cells leading cd44+/cd24+-derived tumors immunoblots showing e-cadherin twist-dysregulated breast cells mcf-10a/twist-shtwist twist+/cd44+/cd24-/low twist+/cd44+/cd24+ subpopulation transient twist-overexpressing cells pmc beta search parental mcf-10a/twist constant-temperature water bath mcf-10a/twist cells cd44+/cd24-/low cells cd44+/cd24-/low cells [35] mcf-7/twist transgenic cells parental mcf-10a cells twist-expressing retroviral constructs pgl4-cd24 reporter construct cd44+/cd24-/low expression canonical e-box sequences twist-overexpressing retroviral constructs cd24 promoter-reporter construct

Questions {❓}

• Breast tumor heterogeneity: cancer stem cells or clonal evolution?
• Normal and leukemic hematopoiesis: are leukemias a stem cell disorder or a reacquisition of stem cell characteristics?

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