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🌠 Phenomenal Traffic: 5M - 10M visitors per month


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Keywords {🔍}

irgm, ptdinsp, doi, fig, pubmed, google, scholar, lipid, class, activity, binding, cells, pik, mpgs, macrophages, protein, rgstirgm, membrane, gtpase, pmc, cell, control, proteins, snapin, ifnγactivated, assays, article, shown, free, supplementary, bcg, targeting, bovis, mycobacterial, min, immunity, infection, recruitment, kinase, inhibition, immunol, helix, overlay, bound, ship, pgs, online, experiments, mpg, tuberculosis,

Topics {✒️}

primary ifn-γ-activated wild-type chemically-treated ifn-γ-activated macrophages 5-bromo-4-chloro-3-indolyl-αd-galactopyranoside 4α-diaza-s-indacene conjugated soluble n-ethylmaleimide-sensitive factor cognate v-snare-expressing compartments primary ifn-γ-activated irgm1+/+ elevated 32p-γ-gtp hydrolysis constructs encoding frb-cfp-inp54p ifn-γ-inducible 47-48kda gtpases ifn-γ-induced phagolysosomal transfer ifn-γ-activated macrophages substantiated catalytically-active g-domain fragment ifn-γ-induced macrophage immunity ifn-γ-inducible irgm1 engages cfp-inp54p-frb bound fkbp alexa594-conjugated anti-mtb complex alexa488-conjugated anti-mtb complex alexa594-conjugated anti-gm130 mab transiently-expressed ha-tagged p21ras ifn-γ-treated irgm1-/- macrophages tyrosine-based sorting motifs lipid-binding mutants egfp-irgm1 dose-dependent rgst-irgm1 binding n-terminal g-domain recombinant mouse ifn-γ burroughs-wellcome fund investigator n-terminal enhanced green ifn-γ-activated macrophage treatment inhibited flag-pik3ca-pik3r1 ifn-γ-inducible lrg-47 15nm bsa-gold preloaded c-terminal αk domain single-turnover gap assays ifn-γ-activated macrophages pik3ca-pik3r1-r2 heterodimers ifn-γ-activated raw264 ifn-γ-induced irgm1 phosphoaminophosphonic acid-guanylate ester17 fkbp-tagged lyn motif replaced specific positively-charged pgbkt7-ci + pact2-ci secondary anti-mouse igg2a heat-inactivated gst-irgm1 served fluorescently-tagged irgm1 variants nonspecific ab cross-reactivity adaptor-complex medium chains specific-pathogen free conditions irrelevant isotype-matched igg fbp-tagged inp54p phosphatase

Questions {❓}

• Are these lipids responsible for MPG recruitment of Irgm1?
• How do enhanced Irgm1 and class I PI(3)K catalytic activities benefit anti-mycobacterial immunity?
• What are the biochemical consequences of Irgm1-PI(3)K interactions?
• What regions of Irgm1 are needed for its recruitment to MPGs?

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