
NCBI . NLM . NIH . GOV {
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Title[redir]:
Direct interaction between Nrf2 and p21Cip1/WAF1 upregulates the Nrf2-mediated antioxidant response - PMC
Description:
In response to oxidative stress, Nrf2 and p21 Cip1/WAF1 are both upregulated to protect cells from oxidative damage. Nrf2 is constantly ubiquitinated by a Keap1 dimer that interacts with a weak-binding 29DLG motif and a strong-binding 79ETGE motif ...
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Keywords {🔍}
nrf, cells, doi, keap, figure, hctp, cell, protein, response, binding, pubmed, google, scholar, expression, dlg, basal, level, oxidative, levels, stress, mefnrf, motif, etge, ubiquitination, antioxidant, domain, pmc, compared, analysis, interaction, residues, conditions, lower, mice, yamamoto, mutants, zhang, induced, activity, pathway, article, transfected, luciferase, proteins, antibodies, panel, performed, control, endogenous, mol,
Topics {✒️}
pulse-chase 35s-labeling method pulse-chase 35s-labeling methods cyclin-dependent kinase inhibitor experimental procedures antibodies pmc beta search residual dose-dependent increase facilitate cullin-mediated ubiquitylation cul3-based e3 ligase double-label indirect immunofluorescence 6-bladed beta-propeller structure pcna-dependent dna replication pulldown 35s-labeled p21 cisplatin-induced dna damage dichlorofluorescein/flow cytometry method ubiquitin-accepting lysine residues nrf2-dependent cytoprotective genes p21-mediated signaling network n-terminal neh2 domain p21-dose-dependent manner 35s-labeled p21 protein redox-sensitive cysteine residues cyclin/cdk binding motifs amino-terminal neh2 domain 35s-labeled nrf2 protein nrf2-dependent antioxidant response p21-dependent cellular protection supplementary materials included p21-dependent cell survival nrf2-mediated antioxidant response gst-nrf2 fusion protein putative p21-interacting proteins strong-binding 79etge motif p21 wild-type mice strong-binding etge motif p21-mediated cell survival keap1-dependent proteasomal degradation gst-p21 fusion proteins weak-binding 29dlg motif ectopically expressed p21-wt induced nrf2-antioxidant response weak-binding dlg motif e3 ubiquitin ligase anti-β-actin antibodies nrf2-keap1 signaling pathway myc-tagged p21 proteins strong 79etge-kelch interaction weak kelch-29dlg binding antioxidant gene regulation reporter gene activity hct116-p21+/+/p21-/- cells
Questions {❓}
- P21(WAF1/Cip1): more than a break to the cell cycle?
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- Ncbi