
NCBI . NLM . NIH . GOV {
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Title[redir]:
Queuosine Formation in Eukaryotic tRNA Occurs via a Mitochondria-localized Heteromeric Transglycosylase - PMC
Description:
tRNA guanine transglycosylase (TGT) enzymes are responsible for the formation of queuosine in the anticodon loop (position 34) of tRNAAsp, tRNAAsn, tRNAHis, and tRNATyr; an almost universal event in eubacterial and eukaryotic species. Despite ...
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Keywords {🔍}
tgt, trna, proteins, google, scholar, pubmed, protein, doi, coli, guanine, mouse, cells, kda, panel, qtrtd, transglycosylase, fig, eukaryotic, subunit, expression, ftgt, enzyme, activity, data, mobilis, variants, found, analysis, sequence, pmc, hand, eubacterial, mitochondria, splice, substrate, acids, observed, biol, article, residues, shown, amino, supplemental, antisera, upper, res, cdna, queuine, rna, qmodification,
Topics {✒️}
accepted form φ-x2–3-φ-x2–3-φ-x-φ isopropyl-1-thio-β-d-galactopyranoside-induced 1 mm isopropyl-1-thio-β-d-galactopyranoside 5-cis-dihydroxy-2-cyclo-penten-1-yl gen-banktm/ebi data bank full-length mouse tgt mouse full-length tgt 7-aminomethyl-7-deazaguanine c-terminal zinc-binding domain n-terminal β1-α1 region c-terminal zinc-binding subdomain predominant splice variant leucine-rich export signal 6′-diamidino-2-phenylindole terminal zinc-binding domain programme grant 05-in3-i761 n-terminally truncated tgt 7-deazapurine-n-glycosidic bond reverse transcription-pcr n-terminal tags interfered post-translational phosphorylation event rna-bound intermediate complexed archaeo-eukaryotic lineages divided nickel-charged hitrap chromatography dna-selective dapi stain c-terminally tagged forms c-terminal subdomain involved ubiquitous dietary-derived component ha-specific monoclonal antibody pmc copyright notice pmc beta search n-terminal ha epitope supplementary materials included c-terminal myc epitope zinc-binding domain gel-filtration studies showed 633-nm laser lines rt-pcr analysis est data bases assess transglycosylase activity zinc-binding subdomain total kidney rna 8 μm [8-14c]guanine gel-filtration chromatography performed immediately c-terminal general acid/base transglycosylase enzyme exists glutamic acid residue active site nucleophile glutathione-agarose
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