
NCBI . NLM . NIH . GOV {
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Title[redir]:
Metastasis-related Plasma Membrane Proteins of Human Breast Cancer Cells Identified by Comparative Quantitative Mass Spectrometry - PMC
Description:
The spread of cancer cells from a primary tumor to form metastasis at distant sites is a complex multistep process. The cancer cell proteins and plasma membrane proteins in particular involved in this process are poorly defined, and a study of the ...
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Keywords {🔍}
expression, cancer, cells, cell, breast, proteins, metastasis, nmc, pubmed, google, scholar, protein, doi, primary, tumor, integrin, membrane, distant, ndrg, lines, analysis, ectont, identified, staining, proteomics, expressed, plasma, data, metastatic, levels, erpr, fold, observed, human, biopsies, hladrα, showed, pmc, higher, compared, tissue, study, level, antibody, fig, normal, tumors, article, mass, clinical,
Topics {✒️}
1 mm l-γ-glutamyl-p-nitroanilide sequential lc-ms/ms analyses tbp/tata box-binding protein fitc-conjugated goat anti-mouse fitc-conjugated swine anti-rabbit hrp-conjugated goat anti-mouse her2-overexpressing subtype er−/pr−/her2+ quantitative lc-ms/ms analysis deglycosylation reagent peptide-n-glycosidase paraffin-embedded tissue blocks polyclonal anti-ecto-5′-nt antibodies motor-driven potter homogenizer m-4a4 versus nm-2c5 nm-2c5 versus m-4a4 fitc-conjugated secondary antibody lc-ms/ms data paraffin-embedded tumor tissue lc-ms/ms silac heat-induced epitope retrieval poor ms/ms spectra site-specific growth potential hrp-conjugated secondary antibody fitc-labeled secondary antibody ecto-5′-nt-expressing subclone pmc beta search plasma/thrombin cell clots major histocompatibility complex γ-glutamyl transpeptidase mass spectrometric-based approaches integrin β1-expressing subclone 2 mg/ml p-iodonitrotetrazolium arf4/adp-ribosylation factor 4 fluorescence-activated cell sorting including hla-dr molecules hla-drb3/hla-drβ3 mda-mb-435 cell line high ecto-5′-nt expression lc-ms/ms eosin-stained tumor slide percoll-sucrose density separation er−/pr− phenotype represented isotope-labeled m-4a4 unlike mhc class mda-mb-435 cell lines hla-drb1/hla-drβ1 cell-surface cd74 initiates expresses melanoma-specific genes paraffin-embedded cells unlabeled nm-2c5 cells comparative quantitative proteomics
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