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Title[redir]:
TTK/hMps1 Mediates the p53-Dependent Postmitotic Checkpoint by Phosphorylating p53 at Thr18 - PMC
Description:
Upon prolonged arrest in mitosis, cells undergo adaptation and exit mitosis without cell division. These tetraploid cells are either eliminated by apoptosis or arrested in the subsequent G1 phase in a spindle checkpoint- and p53-dependent manner. ...
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Keywords {🔍}
cells, ttk, spindle, checkpoint, fig, phosphorylation, thr, pubmed, google, scholar, cell, doi, kinase, ttkhmps, damage, mdm, domain, transfected, protein, ser, western, interaction, response, blotting, activation, pmc, nterminal, mutant, expression, results, biol, postmitotic, dna, proteins, shown, nocodazole, sirna, buffer, article, tetraploid, taxol, assays, material, activity, levels, involved, mitotic, data, arrest, disruption,
Topics {✒️}
pcr-based site-directed mutagenesis anti-phospho-thr18-specific p53 antibody residue-specific phospho-p53 antibodies pmc beta search specific anti-phospho-p53 antibodies n-terminal mdm2-binding domain n-terminal-domain-deleted mutant major n-terminal site anti-cyclin b1 antibody c-terminal tetramerization domain phospho-mimicking p53t18d mutant ttk/hmps1-kd protects p53 n-terminal transactivation domain bacterially produced gst-ttk c-terminal kinase domain sds-polyacrylamide gel electrophoresis gst-fused full-length phospho-mimicking t18d mutant resistant p53-expressing constructs facilitate n-terminal phosphorylation phospho-deficient t18a mutant anti-ttk antibody brought p53 c-terminal domain dna-damage-activated p53 suppressed n-fold increases compared gst-ttk-kd phosphorylated p53 p53 n-terminal domain p53-dependent tetraploid checkpoint n-terminal activation domain p53-dependent postmitotic checkpoint cold phosphate-buffered saline tumor-derived p53 mutants p53 c-terminal phosphorylation ttk/hmps1-downregulated cells treated gst-ttk-kd appeared anti-p53 antibody pab1801 anti-p53 antibody pab421 ttk/hmps1 interacts directly transcription factor composed ttk/hmps1 stabilizes p53 p53-dependent postmitotic arrest dna damage-induced expression tagged full-length p53 ttk-kd protects p53 full-length ha-p53 p21-min reporter carries promotes p53 ubiquitination ttk-kd markedly stabilized n-terminal domain specific upstream activator
Questions {❓}
- How important are post-translational modifications in p53 for selectivity in target-gene transcription and tumour suppression?
- Kinetochore localization of spindle checkpoint proteins: who controls whom?
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