
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization - PMC
Description:
The immortalization of human cells is a critical step during tumorigenesis. In vitro, normal human somatic cells must overcome two proliferative blockades, senescence and crisis, to become immortal. Transformation with viral oncogenes extends the ...
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Keywords {๐}
cells, doi, htert, telomerase, crisis, google, scholar, expression, pubmed, activity, human, htertha, fig, cell, control, telomere, hek, pmc, probe, telomeric, clonal, tagtransformed, senescence, article, populations, dna, transformed, fibroblasts, tag, free, cellular, expressing, clones, immortalization, gene, culture, high, infected, extracts, clone, length, shown, hterthaexpressing, nlm, normal, ectopic, telomeres, growth, isolated, retrovirus,
Topics {โ๏ธ}
t-ag-transformed hek cells blasticidin-resistant vector pwzl-blast human embryonic kidney t-ag-transformed human cells plasmid pci-neo-htert-ha immortalize t-ag-transformed cells htert-ha-transfected hek clones htert-ha-expressing fibroblast clones high htert-ha-expressing clones high htert-ha-expressing clone independent htert-ha-expressing clones terminal restriction fragments t-ag-transformed fibroblasts htert-ha-expressing cells continued pbh-htert-ha plasmid htert-expressing cell lines pmc beta search telomerase-positive cells expressing technology postdoctoral fellowship retroviral htert-ha transcripts htert ecorvโsali fragment htert-ha expression vector htert-ha expression vectors pbp-htert-ha retroviruses htert vector-infected cells ectopically expressed htert-ha hek cells transformed transformed hek cells vector control-infected cells control vector-infected cells transformed human cells studied hek cells transfected htert-ha htert-infected clonal isolates ecoriโsali site human fibroblasts expressing publisher site pdf human somatic cells htert-expressing populations htert permits cells telomerase-negative control cells immortalize human cells htert-expressing retrovirus immortalize transformed cells termed htert-ha t-ag cytosolic cellular extracts htert-expressing retroviruses htert-ha retrovirus terminal growth arrest
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