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Title[redir]:
Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements - PMC
Description:
Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement (βgatingβ current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian ...
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Keywords {π}
vsfp, fluorescence, voltage, currents, figure, doi, gating, cells, current, civsp, vsd, data, yfp, response, steps, cfp, sensor, recorded, membrane, tif, pubmed, google, scholar, fluorescent, channels, charge, activation, expressing, traces, nature, pmc, constructs, potential, amino, signals, single, fit, nlm, measurements, shown, stably, ongating, time, values, protein, cyan, yellow, transient, signal, emission,
Topics {βοΈ}
poly-d-lysine coated coverslips n-methyl-d-glucamine voltage-sensitive fluorescent protein voltage-gated potassium channels ci-vsp voltage sensor ciona intestinalis voltage-sensor pmc beta search voltage-sensing movements cyan/yellow fluorescent protein stronger vsd-fp coupling steady-state relations recorded fast-component cfp response voltage-dependent ion channels fluorescence activation time-constant voltage-sensing mechanisms [5] voltage-sensing domain intrinsic voltage sensor license information pmcid publisher site pdf single exponential fits nih grant ns057631 molecular activation mechanism fluorescent reporter proteins single exponential functions transfected pc12 cells bottom trace shows ascidian ciona intestinalis site-directed mutagenesis fast electrical signals voltage-dependent conformation recorded current trace current trace recorded previously reported vsfp2 voltage command pulses fast cfp component linear leak current recorded current traces fast initial transient activation time-constant 1 voltage-dependent conformational half maximal activation neuronal circuit activity neuronal circuit dynamics fast βgatingβ currents hyperpolarizing voltage step ci-vsp exhibits supplementary materials included dominant time-constant fast reporting vsfp ci-vsp vsd
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