
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Expression of the gut-enriched Krüppel-like factor (Krüppel-like factor 4) gene in the human colon cancer cell line RKO is dependent on CDX2 - PMC
Description:
Gut-enriched Krüppel-like factor (GKLF or KLF4) is a zinc finger-containing, epithelial-specific transcription factor, that functions as a suppressor of cell proliferation. We previously showed that GKLF expression is decreased in intestinal and ...
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Keywords {🔍}
cdx, gklf, doi, cells, scholar, cell, apc, pubmed, google, expression, rko, mutant, wildtype, promoter, pmc, cancer, βcatenin, luciferase, colon, article, lines, figure, free, induction, protein, factor, tcf, mice, promoters, biol, science, gene, line, transcription, intestinal, mutated, costa, activity, human, dang, decreased, tumor, dominant, negative, colorectal, morin, krüppellike, results, kinzler, shields,
Topics {✒️}
β-catenin/tcf-regulated transcription β-catenin-dependent cis-element constitutively active s33y-β-catenin control ht29-β-gal cells constitutively active β-catenin s33y-β-catenin construct wild-type tcf3-luciferase reporters pmc beta search epithelial-specific transcription factor transfected cmv-wild-type cdx2 proposed apc→cdx2→gklf pathway wnt/β-catenin/tcf4 normal-appearing intestinal tissue β-catenin-independent pathway dual-luciferase kit protocol apc→cdx2→gklf→growth arrest s33y-β-catenin significantly ht29-β-gal cells zinc-inducible apc gene entire coding region cdx2-pgl2-luciferase reporter cmv-wild-type cdx2 wild-type tcf3 reporter tcf3 mutant-pgl3-luciferase pgl2-luciferase construct wild-type cdx2 activates da costa lt wild-type cdx2 significantly heterozygous villous colon μg/10-cm dish wild-type tcf3 promoter β-catenin mutations wild-type cdx2 transactivation cmv-renilla luciferase mutant β-catenin wild-type cdx2 protein wild-type apc expression ht29-β-gal adenomatous polyposis coli colon cancer cells constitutively activating mutations β-catenin accumulates references adam pj human mol genet constitutively active protein mutant-cdx2 transfected cells apc decreases c-myc β-gal expression tumor suppressor pathway renilla luciferase assays
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