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Title[redir]:
Structure of an archaeal heterotrimeric initiation factor 2 reveals a nucleotide state between the GTP and the GDP states - PMC
Description:
Initiation of translation in eukaryotes and in archaea involves eukaryotic/archaeal initiation factor (e/aIF)1 and the heterotrimeric initiation factor e/aIF2. In its GTP-bound form, e/aIF2 provides the initiation complex with Met–tRNAiMet. After ...
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Keywords {🔍}
doi, domain, eif, switch, structure, google, scholar, pubmed, fig, ssaifαβγ, pmc, gdp, residues, state, article, conserved, side, free, nucleotide, aifβ, zbd, aifγ, data, initiation, complex, conformation, ion, binding, nterminal, group, γsubunit, factor, eaif, form, start, codon, trna, phosphate, crystallization, magnesium, eukaryotes, heterotrimer, presence, region, protein, central, strictly, site, archaea, aif,
Topics {✒️}
org/cgi/content/full/0706784104/dc1 n-terminal b-α1-helix pmc beta search ss-aif2α3β1γ nucleotide-binding pocket conserved g-219-rsfd-g-222 region remaining c-terminal moiety magnesium-free gdp state n-terminal α1-helix g-148-qnkvd-g-152 motifs eukaryotic/archaeal initiation factor eukaryotic specific n-terminal β-subunit sat astride full trna-binding activity n-terminal α-helix distinct phosphate-binding site long g-l1 loop α1-helix resisted proteolysis conserved g-d309 residue ss-aif2α3βγ heterotrimer highlighting b-α1-helix ensured putative phosphate-binding pocket g-148-qnkvd-g-152 motif g-21-gkt-g-23 loop supporting information online intact ss-aif2αβγ form scanning-competent 48s complex archaeal β-subunits showed conserved g-t46 contacts [google scholar] 41 [google scholar] 42 full aif2αβγ heterotrimer gdp–pi bound state ss-aif2α3βγ electron density present ss-aif2α3βγ structure gdp–pi nucleotide state n-terminal helix archaeal residues g-g96 supplementary materials included gdp–pi state discussed license information pmcid gdp-bound magnesium ion n-terminal helices central α–β domain heterotrimeric initiation factor guanine exchange factor n-terminal part interactions ensured folding heterotrimeric complex showed n-terminal region guanine nucleotide pocket
Questions {❓}
- Can the structure of the above GDP–Pi state be transposed to the case of eukaryotes?
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