
NCBI . NLM . NIH . GOV {
}
Title[redir]:
Novel role for insulin as an autocrine growth factor for malignant brain tumour cells - PMC
Description:
AT/RTs (atypical teratoid/rhabdoid tumours) of the CNS (central nervous system) are childhood malignancies associated with poor survival rates due to resistance to conventional treatments such as chemotherapy. We characterized a panel of human AT/RT ...
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Keywords {🔍}
cells, doi, cell, atrt, insulin, growth, pubmed, google, scholar, proliferation, figure, lines, human, expression, factor, receptor, igfir, signalling, cancer, results, medium, targeting, pik, ini, inhibitor, pmc, mrt, akt, analysis, tumour, protein, kinase, factors, antibodies, analysed, role, article, insulinlike, igfi, gene, activation, autocrine, performed, free, panel, mutations, min, transfected, system, survival,
Topics {✒️}
matrix-assisted laser-desorption ionization–time large-scale rna-interference-based screens high-dose alkylator-based chemotherapy atypical teratoid/rhabdoid tumor atypical teratoid/rhabdoid tumours mammalian swi–snf complex atypical teratoid/rhabdoid tumour atypical teratoid/rhabdoid tumors donkey anti-rabbit igg mammalian swi/snf complexes typical teratoid/rhabdoid tumors penicillin/streptomycin/l-glutamine 2-o-methyl-3-o-octadecylcarbonate] anti-rabbit antibody coupled pmc beta search extracellular-signal-regulated kinase 1/2 anti-β-tubulin antibody long-term insulin stimulation completely inhibited igf-i 1 mm tos-lys-ch2cl serum-free culture conditions western-blot analysis confirmed central nervous system chromatin remodelling factor 1× gel-loading buffer pharmacological akt/pkb inhibitor mitogen-activated protein kinase step rt–pcr kit serum-free supernatants obtained anti-igfir neutralizing antibody pi3k/akt signalling pathway anti-ir neutralizing antibody western-blot analysis revealed phosphoinositide 3-kinase/akt pathway thr202/tyr204-phosphorylated erk malignant rhabdoid tumour rt–pcr analysis revealed malignant rhabdoid tumors class ia pi3k class ia pi3ks insulin activated pi3k/akt ha-tagged ini1 construct pi3k/akt/mtor pathway high-serum culture conditions dominant-negative akt secondary antibodies coupled insulin-stimulated cellular responses license information pmcid tos-lys-ch2cl org/bj/406/bj4060057add
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