
NCBI . NLM . NIH . GOV {
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Title[redir]:
Position of eukaryotic initiation factor eIF5B on the 80S ribosome mapped by directed hydroxyl radical probing - PMC
Description:
Eukaryotic translation initiation factor eIF5B is a ribosome-dependent GTPase that mediates displacement of initiation factors from the 40S ribosomal subunit in 48S initiation complexes and joining of 40S and 60S subunits. Here, we determined ...
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Keywords {🔍}
domain, eifb, cleavage, subunit, rrna, initiation, ribosomal, figure, complexes, hydroxyl, google, scholar, ribosome, pubmed, doi, domains, positions, cleavages, trna, radicals, position, cleaved, binding, surface, eukaryotic, factor, translation, data, similar, free, pmc, joining, structure, radical, pestova, mutants, orientation, eifbs, contacts, δeifb, gtp, allen, supplementary, article, ribosomes, complex, modeled, figures, panel, contact,
Topics {✒️}
70s/mrna/fmet-trnafmet post-initiation complex quaternary 80s/met-trnaimet/mrna/[fe cold spring harbor 80s/met-trnaimet/mrna/[fe pmc beta search eif2·gtp·met-trnaimet bind inability/severely reduced ability potential eif5b-met-trnaimet contact 35 nt-aug-32nt mrna n-terminally truncated δeif5b587–1220 rna-based translation factor thin-layer chromatography analysis local protein-rich environment lanes marked ‘pcl-δeif5b' 43s pre-initiation complex ribosome-dependent gtpase reaction virus ires rna-induced recent cryo-em studies recent cryo-em reconstructions babe]-δeif5b587–1220 quaternary complexes aminoacyl-trna ternary complexes l7/l12 ribosomal proteins methionyl-puromycin synthesis assay saccharomyces cerevisiae–trna–ribosome met-trnaimet base-paired eif5-promoted gtp hydrolysis conserved n-terminal region mock-conjugated pcl-δeif5b587–1220 babe-conjugated wt δeif5b587–1220 induce inter-domain rearrangements babe]-δeif5b587-1220 binary complexes codon–anticodon base-pairing subsequent cryo-em reconstructions slight counter-clockwise rotation designated ‘free aif5b' eif5b n-terminal region ribosome-dependent gtp hydrolysis potential eif5b/80s complex 5′-end labeled met-trnameti pcl-δeif5b587–1220 gtpase activity �free aif5b' structure binary eif5b-80s complexes assembling eif5b/ribosome complexes free archaeal eif5b babe]-δeif5b587–1220 cleavage sites if2 contacts fmet-trnaifmet ribosome-bound if2-gtp 30s subunit a-site fully active pcl-δeif5b587–1220 final eif5b/80s model
Questions {❓}
- Does domain 4 contact initiator tRNA?
- Poyry TA, Kaminski A, Jackson RJ (2004) What determines whether mammalian ribosomes resume scanning after translation of a short upstream open reading frame?
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