
NCBI . NLM . NIH . GOV {
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Title[redir]:
Interleukin 2 gene transcription is regulated by Ikaros-induced changes in histone acetylation in anergic T cells - PMC
Description:
In T cells anergy may be evoked by an unbalanced stimulation of the T-cell receptor in the absence of costimulation. Anergic T cells are unresponsive to new antigen receptor engagement and do not produce interleukin 2. We present evidence that ...
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Keywords {🔍}
cells, doi, ikaros, promoter, expression, anergic, pubmed, google, scholar, anergy, control, figure, anticd, stimulation, tcell, histone, acetylation, ionomycin, shown, induction, cell, chromatin, hours, performed, tsa, immunol, experiments, activation, gene, activity, primers, induce, deacetylation, tcr, complexes, binding, mechanisms, chip, mice, transcription, signaling, specific, antibody, sem, locus, assays, samples, relative, transcriptional, show,
Topics {✒️}
sex-matched pbs-placebo–fed controls rv-ikaros-ires-gfp–transduced cells compared rv-ha-ikaros-ires-gfp virus rv-ha-ikaros1-ires-gfp sex-matched pbs-fed controls retroviral vector rv-ires-gfp ikaros1-expressing plasmid pcdm8-ik-1 plate-bound anti–human cd3 rv-ires-gfp–infected cells plate-bound anti–mouse cd3ϵ rv-gfp–infected control resting rv-ikaros-ires-gfp anti–mouse β-actin antibodies ras/mitogen-activated protein kinase plate-bound anti-cd3 haematopoietic cell-fate decisions quantitative real-time pcr psuper-ik-19i vector pmc beta search psuper-ik-19i constructs dynabeads-cd4 magnetic beads calmodulin-dependent phosphatase calcineurin 20 nm phorbol-12-myristate-13-acetate rv-gfp–infected cells virus expressing ha-ikaros ikaros-expressing retroviral vector murine β-actin gene abnormal t-cell development cn/nfat-dependent manner peripheral t-cell tolerance il2–luciferase reporter plasmid aiolos-deficient mice develop anti–mouse hdac2 antibodies anergy-induced histone deacetylation control pbs-fed mice serum-free medium reactive t-cell activation reactive t-cell receptors induces histone deacetylation graph shows relative gfp-infected control cells ikaros-expressing th1 cells virus expressing gfp pathogen-free conditions deficient h4 deacetylation ikaros-mediated il2 repression murine cd4+cd25+ regulatory anergic t-cell unresponsiveness luciferase reporter plasmid license information pmcid
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