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Title[redir]:
Two Domains of the Progesterone Receptor Interact with the Estrogen Receptor and Are Required for Progesterone Activation of the c-Src/Erk Pathway in Mammalian Cells - PMC
Description:
In breast cancer cells, estrogens activate the Src/Erk pathway through an interaction of the estrogen receptor alpha (ERα) with the SH2 domain of c-Src. Progestins have been reported to activate also this pathway either via an interaction of the ...
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Keywords {🔍}
prb, erα, cells, interaction, activation, csrc, fig, pubmed, google, scholar, eridi, cell, doi, progestins, yeast, eridii, mutants, receptor, pathway, cos, srcerk, domain, assay, lanes, receptors, protein, progesterone, signaling, activity, expression, region, domains, cascade, deletion, vitro, shown, pmc, fusion, proteins, direct, proline, hormone, antibodies, cancer, erk, lysates, panel, cluster, gst, galad,
Topics {✒️}
pmc beta search pcr-mediated site-directed mutagenesis full-length hprb cdna oestradiol-stimulated mcf-7 cells src/ras/erk signaling pathway src/ras/erk signaling cascade vitro-translated 35s-labeled prs src/p21ras/erk pathway full-length erα cdna tris-buffered saline [tbs] sds-polyacrylamide gel electrophoresis unique n-terminal segment 1 mm isopropyl-β-thiogalactopyranoside src/ras/erk cascade progesterone-responsive reporter gene endogenous src/erk pathway 35s-labeled human prb endogenous src/erk cascade src/erk signaling pathway prostate cancer cells sequence-specific transcription factors mitogen-activated protein 35s-labeled pr mutants mmtv-luc reporter gene n-terminal inhibitory function progesterone-responsive reporter genes c-src/erk pathway 35s-labeled prb produced vectors expressing wild-type wild-type prb coimmunoprecipitated gal4ad-prmpro fusion protein ligand-dependent transactivation domain gst-erαlbd fusion protein human breast cancer mmtv-luc reporter plasmid gal4ad-prb fusion protein progesterone-induced maturation differential hormone-dependent phosphorylation src remains unclear glutathione-sepharose 4b beads estrogen receptor-mediated transcription gal4dbd-erα fusion protein breast cancer res glutathione s-transferase map kinase pathway map kinase cascade rabbit reticulocyte lysate gal4ad-prb confers histidine breast cancer cells protein-coupled receptors
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