
NCBI . NLM . NIH . GOV {
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Title[redir]:
Human Dcp2: a catalytically active mRNA decapping enzyme located in specific cytoplasmic structures - PMC
Description:
We have cloned cDNAs for the human homologues of the yeast Dcp1 and Dcp2 factors involved in the major (5′–3′) and NMD mRNA decay pathways. While yeast Dcp1 has been reported to be the decapping enzyme, we show that recombinant human Dcp2 (hDcp2) is ...
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Keywords {🔍}
hdcp, dcp, mrna, decapping, yeast, protein, activity, human, rna, decay, cells, proteins, pubmed, google, scholar, doi, cell, figure, pbs, cap, recombinant, lanes, active, enzyme, domain, specific, pathway, reaction, free, pmc, conserved, product, vitro, substrate, presence, degradation, sequence, data, shown, purified, structures, muttnudix, mrnas, expression, article, products, cytoplasm, cterminal, control, translation,
Topics {✒️}
expressed full-length his6-tagged-hdcp1 full-length gst–hdcp2-his6 purified rabbit anti-hdcp1 serum his6–gst-tagged hdcp1δc pmc beta search glutathione–sepharose 4b beads unrelated his6–gst fusion full-length protein devoid mutt/nudix domain similar long c-terminal tails c-terminal his6 tag conserved c-terminal tail conserved nudix/mutt domain 20 mm hepes–koh ph 7 n-terminal gst tag divergent c-terminal tail 50 mm tris–hcl ph 7 45 mm tris–hcl ph 8 1093/emboj/cdf678 human dcp2 nucleotide di-phosphate kinase nuclear pre-mrna turnover 132 µm [α-32p]gtp conserved n-terminal domain full-length yeast dcp2 background cap-cleaving activity purified gst–hdcp2-his6 conserved n-terminal moiety yfp-hdcp1-expressing cell full-length proteins obtained full-length version indicating cap-labelled rna digested gst–hdcp2-his6 protein full-length hdcp2 protein yfp-hdcp2-expressing cell cap-labelled rna substrate yfp-hsnu30-2 splicing factor time-couse experiment showed radiolabelled free cap pei-cellulose tlc plates peyfp-c1 plasmids free phosphate due gene-disrupted yeast cells highly conserved mutt c-terminal extensions glutamic acid substitution full-length cdna c-terminal tail cfp-hdcp1 expression contructs coomassie blue-stained gel gfp-hdcp1 expression plasmid
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