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Keywords {🔍}

tada, trna, trnaarg, figure, position, mutant, pubmed, google, scholar, lane, doi, yeast, protein, anticodon, ecoli, rna, gene, shown, tadp, activity, vitro, trnas, sequence, recombinant, deaminase, pmc, editing, deamination, gerber, loop, buffer, proteins, adenosine, incubated, formation, results, article, wobble, cells, lanes, free, essential, substrate, trnaala, deaminated, nwl, minisubstrate, arg, reaction, strain,

Topics {✒️}

uniformally [α-33p]atp-labeled trnaarg2 adding isopropyl-β-d-galactopyranoside [33p]atp-labeled trna pmc beta search [33p]atp-labeled trnaarg2 ni2+-nta–agarose previously equilibrated u5] tri-snrnp protein chemical cross-linking experiments efficient cross-linking triggered uv-cross-linking experiments mouse anti-flag antibody synonymous u-starting anticodon putative zn2+-chelating residues 50 mm tris–hcl ph 8 25 mm tris–hcl ph 8 33p-labeled trna substrate trna-specific adenosine deaminase position-specific gap penalties wild-type growth phenotype plasmid-borne tada gene pre-mrna editing enzymes sds–gel loading buffer chemical cross-linking cold spring harbor wild-type chromosomal copy 24 pmol/µl t7 primer empty-vector-transformed cells plasmid-encoded chloramphenicol resistance site-specific inosine formation coli enzyme tada/ecadat2 musier-forsyth recombinant flag-tada-his6 tris–hcl ph 8 ura3-marked tad2 plasmid 20 pmol/µl substrate primer codon–anticodon base pairing 35s-labeled tada hepatitis delta virus cell-killing function encoded flag-tagged recombinant tada ni2+-nta–agarose cross-linking reaction zn2+-chelating residues find similar numbers kanamycin-disrupted tada gene uv cross-linked 12% sds–polyacrylamide gel 15% sds–polyacrylamide gel antibiotic-resistance profile pre-mrna editing

Questions {❓}

• (1998) Diverse RNA substrates for aminoacylation: clues to origins?
• (2001) A primordial tRNA modifiaction required for the evolution of life?

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