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Title[redir]:
Modification of the human thymine-DNA glycosylase by ubiquitin-like proteins facilitates enzymatic turnover - PMC
Description:
DNA glycosylases initiate base excision repair (BER) through the generation of potentially harmful abasic sites (AP sites) in DNA. Human thymine-DNA glycosylase (TDG) is a mismatch-specific uracil/thymine-DNA glycosylase with an implicated function ...
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Keywords {🔍}
tdg, sumo, dna, protein, proteins, human, glycosylase, modified, pubmed, google, scholar, doi, buffer, site, substrate, modification, beads, sumoylation, cells, hela, recombinant, figure, extract, ape, nonmodified, min, interaction, nuclear, repair, pmc, base, binding, affinity, western, hardeland, conjugation, glycosylases, reaction, antibody, specific, processing, incubated, lanes, blotting, incubation, free, biol, article, enzyme, activity,
Topics {✒️}
mismatch-specific uracil/thymine-dna glycosylase purified his6-tagged wild-type mismatch-specific thymine-dna glycosylase mismatch-specific dna n-glycosylase protein-coated affi-gel beads recombinant his6-tagged tdg equilibrated ni-nta–agarose pmc beta search uracil-dna glycosylases related polyclonal anti-tdg antibody ni2+-nta beads coated transactivator protein ie2-p86 n-glycosidic bond connecting transcriptional transactivator ie2-p86 monoclonal anti-sumo-1 antibody polyclonal anti-sumo-2/3 antibody human thymine-dna glycosylase mismatch-specific dna glycosylase 120 mm tris–hcl ph 6 50 mm tris–hcl ph 8 tdg-specific monoclonal antibody dna base excision-repair cysteine protease-sensitive modification smug1 uracil-dna glycosylase tdg-specific polyclonal antibody 50 mm na-phosphate ph 8 human 8-oxoguanine-dna glycosylase cell-cycle-dependent assembly dna-binding affinity imposed activated water molecule affi-gel®10 beads coated human uracil-dna glycosylase major tdg-specific signals modulate protein–protein interactions single c-terminal lysine ni-nta–agarose damage-specific dna glycosylases tdg-specific monoclonal antibodies thymine-dna glycosylase thymine dna glycosylase covalent post-translational modification bsa-coated affinity beads monoclonal antibody directed double-stranded dna license information pmcid base excision repair modification-deficient k330a mutant ni-nta eluate gel-recovered modified tdg ap-endonuclease acting downstream
Questions {❓}
- What possible role can we assign to sumoylation of TDG on the basis of the obvious changes induced in its enzymatic properties?
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