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Title[redir]:
Disruption of the Uty epigenetic regulator locus in hematopoietic cells phenocopies the profibrotic attributes of Y chromosome loss in heart failure - PMC
Description:
Heart failure affects millions of people worldwide, with men exhibiting a higher incidence than women. Our previous work has shown that mosaic loss of the Y chromosome (LOY) in leukocytes is causally associated with an increased risk for heart ...
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Keywords {🔍}
cells, mice, cardiac, genes, fig, data, chromosome, utygt, gene, analysis, heart, loy, tac, uty, control, hematopoietic, macrophages, cell, expression, doi, pmc, article, pubmed, google, scholar, extended, wildtype, weight, blood, bone, marrow, free, leukocytes, monocytes, test, singlecell, xyx, disruption, failure, loss, dysfunction, mouse, cat, performed, days, chromatin, model, tissue, hearts, revealed,
Topics {✒️}
org/gpp/public/analysis-tools/sgrna-design multi-modal single-cell omics transforming growth factor-β clinical single-cell rna-sequencing remove free-floating cells crispr/cas9-mediated gene disruption ddx3y-deficient mice b6d2-ddx3yem2osb cd45−cd31−mef−sk4+ fibroblasts single-cell multiomics dataset 27-gauge blunt-needle ligation phase separation-mediated regulation identifying chip–seq enrichment efficient crispr-mediated mutagenesis multimodal single-cell data multimodal single-cell analysis single-stranded sgrna sequences integrated scrna-seq datasets post-bone marrow transplant fluorescence-activated cell sorting pmc beta search cd45−cd31+mef−sk4− cd45−cd31−mef−sk4+ previous scrna-seq datasets wnt5a-mediated neutrophil recruitment generated scrna-seq database bone marrow-derived macrophage transcription factor-binding sequences anti-tgfβ antibody prevented crispr/cas9-mediated disruption crispr/cas9-based screen tet2-mediated clonal hematopoiesis bone marrow-derived macrophages rosa-cas9 knockin mice21 underpin loy-related pathologies utygt-mediated cardiac dysfunction transposase-accessible chromatin sequencing shapiro–wilk normality test impaired long-term survival hematopoietic stem/progenitor cells posterior wall thickness single-cell multiomics uty-knockout mouse strain cd115−ly6g−cd3e+cd4+ cd115−ly6g−cd3e+cd8+ structural gene y-linked cd45+cd64+ly6g−ly6clow circulating monocyte-derived macrophages single-nucleus profiling repeated-measures anova cd45+cd64+ly6g−ly6chi
Questions {❓}
- This observation prompts additional questions: could similar epigenetic changes take place at even earlier stages, such as within progenitor cells or hematopoietic stem cells?
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