
NCBI . NLM . NIH . GOV {
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Title[redir]:
Cloning and Characterization of a Novel Hepatitis B Virus x Binding Protein That Inhibits Viral Replication - PMC
Description:
The hepatitis B virus and the mammalian hepadnavirus genomes encode for a short open reading frame called x. Expression of the protein product (HBx) appears necessary for establishment of natural infection. However, in vitro studies have suggested a ...
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Keywords {🔍}
hbx, xip, protein, doi, hbv, cells, virus, replication, hepatitis, expression, cell, pubmed, google, scholar, viral, pmc, cdna, levels, payw, article, transactivation, free, vector, dna, hepg, binding, yeast, plasmid, fig, luciferase, wildtype, orf, transfected, cellular, mutant, site, virol, transfection, interact, activity, shown, jvi, transcription, bait, hcc, interaction, proteins, analysis, line, human,
Topics {✒️}
5-bromo-4-chloro-3-indolyl-β-d- galactopyranoside c-jun/c-fos heterodimers 12-o-tetradecanoyl-phorbol-13-acetate induction immunoprecipitate ivt [35s]methionine-hbx ivt [35s]methionine-labeled hbx ivt [35s]methionine-hbx binding nf-κb transcription factors full-length hbx proved full-length dhbv probe include carboxy-terminal deletions carboxy-terminally truncated hbx [35s]methionine ivt luciferase hbx-minus variant strain pmc beta search upregulate ap-1-responsive elements anti-lexa polyclonal antibody expressed wild-type hbv virus enhancer/promoter elements myc-lexa fusion proteins replication-competent dhbv genome pooled puromycin-resistant bphepg2 hbv core promoter/enhancer xip-encoding cdna clone prepare high-affinity antibodies basic region-leucine domain pmc copyright notice direct x-gal staining retrovirus-transduced cell lines m2 anti-flag mab anti-flag m2 mab yeast-derived bridging protein false-positive yeast colonies full-length hbx lexa-hbx fusion protein anti-hbx mab immunoprecipitated wild-type hbv payw1 c-terminal truncations hbx-minus viral species hbx activated transcription wild-type plasmid payw1 p-73colluc reporter plasmid hbx-minus plasmid payw7 whv-infected woodchuck liver cytoplasm-derived nucleocapsid particles false-positive interaction due single yeast-resistant colonies virus x-gene product gst-xip fusion protein lexa-hbx bait constructs carboxy-terminal deletions
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