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🌠 Phenomenal Traffic: 5M - 10M visitors per month


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Keywords {🔍}

cells, doi, degradation, cell, fig, protein, pubmed, google, scholar, surface, lytac, cul, egfr, min, pmc, article, free, cimpr, hours, membrane, data, proteins, ctx, treatment, ctxmpn, genes, lysosomal, incubated, analysis, proteomics, washed, flow, lytacmediated, lytacs, trafficking, neddylation, determined, cytometry, receptor, retromer, umrc, internalization, igg, extracellular, complex, pbs, beads, peptides, mass, buffer,

Topics {✒️}

n-acetylglucosamine-1-phosphodiester alpha-n-acetylglucosaminidase genome-wide crispr-cas9 screen genome-wide single-guide rna ci-m6pr–lytac–mediated lysosomal trafficking supplementary checklist nihms1952705-supplement-supplementary_checklist functional ci-m6pr–mediated transport org/searleb/encyclopedia/wiki/home genome-wide crispr screen ci-m6pr–mediated lysosomal transport lonza 4d-nucleofector unit m6p–n-glycan biosynthesis target-lytac–ci-m6pr complexes genome-wide crispr knockout high-energy collision–induced dissociation cell-impermeable nhs-sulfo-biotin triantennary n-acetylgalactosamine conjugates k-ɛ-gg peptides biotage snap kp-sil recycling lytac-ci-m6pr complexes copper-free click chemistry proton-decoupled carbon-13 nmr solid-phase peptide synthesis data-dependent ms/ms scans n-acetylglucosamine-1-phosphate transferase lc-ms/ms analysis library-free peptide detection m6pn5-conjugated goat-anti-rabbit genome-wide screen confirmed k-ɛ-gg improve lytac-mediated degradation cytokine receptor–targeting chimeras ci-m6pr–mediated internalization figures nihms1952705-supplement-supplementary_text_and_figures cell surface ci-m6pr ci-m6pr–induced degradation lytac–ci-m6pr complexes ci-m6pr–lytac complexes alanine–serine–mannose 6–phosphonate surface ci-m6pr expression lytac–ci-m6pr complex altered ci-m6pr occupancy crispr-mediated gene knockout increased unoccupied ci-m6pr chemical synthesis procedures higher-affinity m6p-ligand data nihms1952705-supplement-data nedd-cul1 conjugate cleavage discrete m6p-tagged proteins lytac-mediated target maturation fluorophore-labeled rabbit immunoglobulin

Questions {❓}

• Braulke T, Gartung C, Hasilik A, von Figura K, Is movement of mannose 6-phosphate-specific receptor triggered by binding of lysosomal enzymes?
• Von Heijne G, The membrane protein universe: What’s out there and why bother?

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• https://www.linkedin.com/company/ncbinlm
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• https://www.youtube.com/user/NLMNIH

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