BIORXIV . ORG {
}
Title:
A Statistical Framework for Robust and Reproducible BioImage Analysis | bioRxiv
Description:
Bioimage analysis is a powerful tool for investigating complex biological processes, but its robustness depends on technical precision and rigorous experimental design. In particular, the use of appropriate controls and experimental repetition is critical for drawing meaningful conclusions. However, both are often used inadequately or overlooked, with “statistical significance” often prioritised, frequently obtained through the misuse or misinterpretation of statistical tests. In this study, we reanalyse publicly available image datasets to highlight the crucial role of robust experimental design in interpreting results. Our findings underscore the importance of focusing on effect sizes and biological relevance over arbitrary statistical thresholds. We also discuss the diminishing returns of increased data collection once statistical stability has been achieved. By refining control usage and emphasising effect sizes, this work aims to enhance the reproducibility and robustness of research findings. We provide open-access code to allow researchers to engage with the dataset, promoting better practices in experimental design and data interpretation. ### Competing Interest Statement The authors have declared no competing interest.
Website Age:
12 years and 2 months (reg. 2013-04-08).
Matching Content Categories {📚}
- Education
- Science
- Technology & Computing
Content Management System {📝}
What CMS is biorxiv.org built with?
Website use Drupal 7 (http://drupal.org).
Traffic Estimate {📈}
What is the average monthly size of biorxiv.org audience?
💥 Very Strong Traffic: 200k - 500k visitors per month
Based on our best estimate, this website will receive around 261,717 visitors per month in the current month.
check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush
How Does Biorxiv.org Make Money? {💸}
We're unsure how the site profits.
Websites don't always need to be profitable; some serve as platforms for education or personal expression. Websites can serve multiple purposes. And this might be one of them. Biorxiv.org has a revenue plan, but it's either invisible or we haven't found it.
Keywords {🔍}
biology, statistical, view, orcid, profile, experimental, medrxiv, subject, reviews, clinical, results, preprint, design, biorxiv, university, trials, epidemiology, search, robust, bioimage, analysis, pdf, biological, robustness, findings, effect, sizes, data, research, competing, interest, authors, copyright, license, institute, technology, cancer, cell, context, genetics, microbiology, papers, submitted, skip, main, content, homesubmitfaqblogalerts, rssresourcesaboutchannels, keyword, advanced,
Topics {✒️}
epidemiology subject categories provide open-access code clinical trials biorxiv subject category genetics microbiology increased data collection reviews epidemiology papers author/funder keyword advanced search health-related information data interpretation drawing meaningful conclusions refining control usage generous financial support chan zuckerberg initiative imperial college london vrije universiteit amsterdam articles animal behavior arbitrary statistical thresholds rigorous experimental design emphasising effect sizes competing interest statement context robust experimental design experimental design effect sizes biological relevance research findings competing interest experimental repetition statistical framework �statistical significance” statistical tests statistical stability results follow powerful tool technical precision frequently obtained reanalyse publicly image datasets crucial role interpreting results findings underscore diminishing returns work aims california institute massachusetts institute top previous
Social Networks {👍}(4)
- https://www.biorxiv.org/content/10.1101/2025.02.10.637409v2/highwire_log/share/twitter?link=http%3A%2F%2Ftwitter.com%2Fshare%3Furl%3Dhttps%253A%2F%2Fwww.biorxiv.org%2Fcontent%2F10.1101%2F2025.02.10.637409v2%26text%3DA%2520Statistical%2520Framework%2520for%2520Robust%2520and%2520Reproducible%2520BioImage%2520Analysis
- https://www.biorxiv.org/content/10.1101/2025.02.10.637409v2/highwire_log/share/facebook?link=http%3A%2F%2Fwww.facebook.com%2Fsharer.php%3Fu%3Dhttps%253A%2F%2Fwww.biorxiv.org%2Fcontent%2F10.1101%2F2025.02.10.637409v2%26t%3DA%2520Statistical%2520Framework%2520for%2520Robust%2520and%2520Reproducible%2520BioImage%2520Analysis
- https://www.biorxiv.org/content/10.1101/2025.02.10.637409v2/highwire_log/share/linkedin?link=http%3A%2F%2Fwww.linkedin.com%2FshareArticle%3Fmini%3Dtrue%26url%3Dhttps%253A%2F%2Fwww.biorxiv.org%2Fcontent%2F10.1101%2F2025.02.10.637409v2%26title%3DA%2520Statistical%2520Framework%2520for%2520Robust%2520and%2520Reproducible%2520BioImage%2520Analysis%26summary%3D%26source%3DbioRxiv
- http://twitter.com/share?url=https%3A//www.biorxiv.org/content/10.1101/2025.02.10.637409v2&count=horizontal&via=&text=A%20Statistical%20Framework%20for%20Robust%20and%20Reproducible%20BioImage%20Analysis&counturl=www.biorxiv.org/content/10.1101/2025.02.10.637409v2
External Links {🔗}(9)
- What's the income of http://orcid.org/0000-0003-2763-5244?
- What's the revenue for http://orcid.org/0000-0002-2877-0133?
- How much does http://orcid.org/0009-0003-1942-6407 pull in monthly?
- How much does http://orcid.org/0000-0001-7219-560X make?
- Monthly income for https://github.com/FrancisCrickInstitute/Enhancing-Reproducibility
- How much revenue does http://creativecommons.org/licenses/by/4.0/ bring in?
- How much does https://submit.medrxiv.org generate monthly?
- https://web.hypothes.is/'s financial summary
- How much revenue does https://www.altmetric.com/ generate?
Analytics and Tracking {📊}
- Google Analytics
- Google Analytics 4
- Google Tag Manager
Libraries {📚}
- Clipboard.js
- jQuery
- Modernizr
Emails and Hosting {✉️}
Mail Servers:
- usb-smtp-inbound-1.mimecast.com
- usb-smtp-inbound-2.mimecast.com
Name Servers:
- jen.ns.cloudflare.com
- vin.ns.cloudflare.com
CDN Services {📦}
- Jsdelivr