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Cell death in Leishmania induced by stress and differentiation: programmed cell death or necrosis? | Cell Death & Differentiation
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Unicellular organisms, such as the protozoan parasite Leishmania, can be stimulated to show some morphological and biochemical features characteristic of mammalian apoptosis. This study demonstrates that under a variety of stress conditions such as serum deprivation, heat shock and nitric oxide, cell death can be induced leading to genomic DNA fragmentation into oligonucleosomes. DNA fragmentation was observed, without induction, in the infectious stages of the parasite, and correlated with the presence of internucleosomal nuclease activity, visualisation of 45 to 59 kDa nucleases and detection of TUNEL-positive nuclei. DNA fragmentation was not dependent on active effector downstream caspases nor on the lysosomal cathepsin L-like enyzmes CPA and CPB. These data are consistent with the presence of a caspase-independent cell death mechanism in Leishmania, induced by stress and differentiation that differs significantly from metazoa.
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cell, death, activity, dna, figure, pubmed, article, google, scholar, leishmania, cas, nuclei, fragmentation, parasites, lane, nuclease, phase, promastigotes, observed, caspase, mexicana, amastigote, apoptosis, presence, shown, data, cells, amastigotes, major, nuclear, degradation, mnase, addition, apoptotic, differentiation, parasite, programmed, mouse, cysteine, devdase, incubated, pcd, chromatin, stationary, logarithmic, nucleases, axenics, days, min, assay,
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nature portfolio permissions reprints privacy policy mexicana mnyc/bz/62/m379 wild-type nature advertising author information authors caspase-3-7 substrate ac-devd-amc cysteine proteinase-deficient mutants author correspondence unique c-terminal extension host-parasite interactions curr chromosome-scale genome sequencing sds–dna–page system sds–dna–page technique 30 μm ac-devd-amc full size image permissions δcpa/cpb mutant39 promastigotes post-mortem event due ca2+-specific chelator egta development welburn sc caspase inhibitor z-vad mouse lesion isolated-amastigotes mouse lesion-isolated amastigotes 1 mg/ml ethidium bromide tunel-positive parasite nuclei trypanosoma brucei rhodesiense elongation factor 1-alpha access endogenous site-unspecific endonuclease social media cell death differ cysteine proteinase mol culture media tunel-positive amastigote nuclei cpb null mutants lysosomal cysteine proteases recombinant cysteine proteinase unicellular organism leishmania generalised visceral infection glass slides covered transmission electron microscopy olie ra belli si wild-type axenics appeared short heat-shock treatment brucei procyclic forms human parasite leishmania
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- 1996 A new twist to the tale?
- Cell death in Leishmania induced by stress and differentiation: programmed cell death or necrosis?
- Cell death in Leishmania induced by stress and differentiation: programmed cell death or necrosis?
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