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Title:
Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins - PubMed
Description:
Many cellular events depend on a tightly compartmentalized distribution of H+ ions across membrane-bound organelles. However, measurements of organelle pH in living cells have been scarce. Several mutants of the Aequorea victoria green fluorescent protein (GFP) displayed a pH-dependent absorbance an …
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Keywords {🔍}
doi, pmc, pmid, cells, fluorescent, pubmed, green, proteins, protein, figure, free, article, liu, states, mitochondrial, biol, nih, articles, cited, golgi, living, hela, cell, jan, hhsunited, main, ncbi, similar, references, mesh, resources, pnas, measurement, medialtransgolgi, subcellular, microscopy, membrane, fluorescence, biophys, zhang, apr, review, luminescent, ecollection, metabolism, hhs, page, content, homepage, navigation,
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k26r/f64l/s65t/y66w/n146i/m153t/ v163a/n164h/h231l united states government funding linkout main page content green fluorescent proteins similar articles cited mutations f64l/s65t/h231l hhs green fluorescent protein s65g/s72a/t203y/h231l articles references schmidt monophenyl luminescent material fluorescent protein biosensors medial/trans-golgi counter-ion literature sources membrane-bound organelles cytosol golgi membrane hela cells ph-dependent absorbance context-dependent activation 6803 abstract unravelling molecular dynamics single living cells cellular events depend tightly compartmentalized distribution expected subcellular localization rat neonatal cardiomyocytes specific subcellular locations sites previously inaccessible compartment-specific expression visualizing subcellular organelles quantitative fluorescence microscopy cloflucarban illuminates specificity pink1-parkin pathway reported local ph ph-sensitive sensors fluorescent emission golgi ph cited mitochondrial complex inhibition organelle ph detection dual-state emission trends cell biol mol biol cell mitochondrial matrix ph research support pubmed ohkuma pubmed chacon
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