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Title:
Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins - PubMed
Description:
Many cellular events depend on a tightly compartmentalized distribution of H+ ions across membrane-bound organelles. However, measurements of organelle pH in living cells have been scarce. Several mutants of the Aequorea victoria green fluorescent protein (GFP) displayed a pH-dependent absorbance an …
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Keywords {🔍}
doi, pmc, pmid, pubmed, cells, fluorescent, green, proteins, free, article, mitochondrial, protein, figure, biol, states, review, nih, articles, cited, jun, golgi, microscopy, hela, hhsunited, main, ncbi, abstract, similar, references, mesh, resources, sci, measurement, living, organelle, medialtransgolgi, subcellular, fluorescence, biophys, mar, cell, metabolism, hhs, page, content, homepage, navigation, search, full, text,
Topics {✒️}
k26r/f64l/s65t/y66w/n146i/m153t/ v163a/n164h/h231l united states government funding linkout main page content green fluorescent proteins similar articles cited mutations f64l/s65t/h231l hhs s65g/s72a/t203y/h231l green fluorescent protein articles references schmidt medial/trans-golgi counter-ion literature sources books medgen grants ph-dependent absorbance cytosol hela cells protein damage susceptibility quantitative fluorescence microscopy single living cells cellular events depend tightly compartmentalized distribution membrane-bound organelles expected subcellular localization rat neonatal cardiomyocytes specific subcellular locations sites previously inaccessible compartment-specific expression visualizing subcellular organelles trends cell biol v-atpase activity phosphatidylinositol phosphate lipids mol biol cell golgi membrane reported local ph ph-sensitive sensors fluorescent emission cited golgi ph organelle ph detection mitochondrial matrix ph research support 6803 abstract abstract living cells pubmed ohkuma pubmed chacon pubmed wordmark pubmed logo
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