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We are analyzing https://www.nature.com/articles/s42003-020-0763-1.

Title:
Recurrent pregnancy loss is associated with a pro-senescent decidual response during the peri-implantation window | Communications Biology
Description:
During the implantation window, the endometrium becomes poised to transition to a pregnant state, a process driven by differentiation of stromal cells into decidual cells (DC). Perturbations in this process, termed decidualization, leads to breakdown of the feto-maternal interface and miscarriage, but the underlying mechanisms are poorly understood. Here, we reconstructed the decidual pathway at single-cell level in vitro and demonstrate that stromal cells first mount an acute stress response before emerging as DC or senescent DC (snDC). In the absence of immune cell-mediated clearance of snDC, secondary senescence transforms DC into progesterone-resistant cells that abundantly express extracellular matrix remodelling factors. Additional single-cell analysis of midluteal endometrium identified DIO2 and SCARA5 as marker genes of a diverging decidual response in vivo. Finally, we report a conspicuous link between a pro-senescent decidual response in peri-implantation endometrium and recurrent pregnancy loss, suggesting that pre-pregnancy screening and intervention may reduce the burden of miscarriage. Lucas, Vrljicak et al characterise how the cycling endometrium transitions to a pregnant state. Through single-cell analysis of endometrial stromal cells and timed endometrial biopsies they identify DIO2 and SCARA5 as markers of a divergent decidual response.
Website Age:
30 years and 10 months (reg. 1994-08-11).

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Keywords {🔍}

cells, pubmed, decidual, genes, article, google, scholar, cas, analysis, cell, endometrial, expression, fig, sndc, unk, ensc, scara, senescence, gene, response, dio, data, decidualization, human, pregnancy, stromal, endometrium, biopsies, supplementary, central, singlecell, senescent, rpl, control, marker, nature, loss, pathway, stress, levels, networks, test, performed, including, recurrent, cellular, decidualizing, subjects, implantation, immune,

Topics {✒️}

nature portfolio circ-hdac4/mir-30c/rbpj axis privacy policy multi-omics-based mapping body mass index c-chip fuchs-rosenthal haemocytometer c-jun n-terminal kinase duoset elisa kits advertising bead solutions interferon-gamma modulates prolactin thermofisher scientific nature 509 nature 563 nature phenol red-free dmem/f-12 reprints determine library size open arrows high-throughput rna-sequencing34 dmem-f12 media supplemented post-k-pg radiation k-means cluster analysis potent anti-inflammatory glucocorticoid reactive oxygen species select high-quality data dedicated research clinic implantation research clinic 12 µl nuclease-free water tagmented libraries drop-seq data alignment eluted libraries functional decidual–placental interface single-cell rna-seq aid unk-cell maturation additional single-cell analysis library dilution magnetic-activated cell sorting magnetic-activated cell separation peri-implantation endometrial biopsies conjugated anti-cd56 antibody enzyme-linked immunosorbent assay uterine natural killer pro-senescent decidual response single-cell transcriptomic analysis stroma-specific marker genes hematoxylin staining – stromal cells 1x antibiotic-antimycotic mix pro-senescent decidual state permanent cell-cycle arrest

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      headline:Recurrent pregnancy loss is associated with a pro-senescent decidual response during the peri-implantation window
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