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Title:
COMRADES determines in vivo RNA structures and interactions | Nature Methods
Description:
The structural flexibility of RNA underlies fundamental biological processes, but there are no methods for exploring the multiple conformations adopted by RNAs in vivo. We developed cross-linking of matched RNAs and deep sequencing (COMRADES) for in-depth RNA conformation capture, and a pipeline for the retrieval of RNA structural ensembles. Using COMRADES, we determined the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs. In vivo probing of RNA structures with COMRADES yields insight into RNA folding of the ZIKA virus genome and its interaction with host RNAs.
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headline:COMRADES determines in vivo RNA structures and interactions
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Isolation, separation and purification
Retrovirus
RNA-binding proteins
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RNA metabolism
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Biological Microscopy
Biomedical Engineering/Biotechnology
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headline:COMRADES determines in vivo RNA structures and interactions
description:The structural flexibility of RNA underlies fundamental biological processes, but there are no methods for exploring the multiple conformations adopted by RNAs in vivo. We developed cross-linking of matched RNAs and deep sequencing (COMRADES) for in-depth RNA conformation capture, and a pipeline for the retrieval of RNA structural ensembles. Using COMRADES, we determined the architecture of the Zika virus RNA genome inside cells, and identified multiple site-specific interactions with human noncoding RNAs. In vivo probing of RNA structures with COMRADES yields insight into RNA folding of the ZIKA virus genome and its interaction with host RNAs.
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Genomic analysis
Isolation, separation and purification
Retrovirus
RNA-binding proteins
RNA folding
RNA metabolism
RNA sequencing
Structure determination
Life Sciences
general
Biological Techniques
Biological Microscopy
Biomedical Engineering/Biotechnology
Bioinformatics
Proteomics
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