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We are analyzing https://www.nature.com/articles/s41563-021-01136-7.

Title:
Synthetic dynamic hydrogels promote degradation-independent in vitro organogenesis | Nature Materials
Description:
Epithelial organoids are most efficiently grown from mouse-tumour-derived, reconstituted extracellular matrix hydrogels, whose poorly defined composition, batch-to-batch variability and immunogenicity limit clinical applications. Efforts to replace such ill-defined matrices for organoid culture have largely focused on non-adaptable hydrogels composed of covalently crosslinked hydrophilic macromolecules. However, the excessive forces caused by tissue expansion in such elastic gels severely restrict organoid growth and morphogenesis. Chemical or enzymatic degradation schemes can partially alleviate this problem, but due to their irreversibility, long-term applicability is limited. Here we report a family of synthetic hydrogels that promote extensive organoid morphogenesis through dynamic rearrangements mediated by reversible hydrogen bonding. These tunable matrices are stress relaxing and thus promote efficient crypt budding in intestinal stem-cell epithelia through increased symmetry breaking and Paneth cell formation dependent on yes-associated protein 1. As such, these well-defined gels provide promising versatile matrices for fostering elaborate in vitro morphogenesis. The influence of stress relaxation of the extracellular matrix on the formation of intestinal organoids was investigated. It was shown that a stress-relaxing synthetic matrix promotes crypt budding through increased symmetry breaking and niche cell formation.
Website Age:
30 years and 10 months (reg. 1994-08-11).

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article, google, scholar, data, cas, nature, hydrogels, organoid, gels, organoids, intestinal, cell, hybrid, culture, stem, mater, analysis, covalent, cells, hsi, shown, lutolf, access, nat, stress, development, extended, content, synthetic, research, hydrogel, adv, relaxation, line, fig, budding, matrigel, expression, independently, prepared, curve, points, information, vitro, grown, human, lausanne, gel, strain, representative,

Topics {✒️}

nature portfolio journals permissions reprints research bio-inspired materials nature portfolio privacy policy nucleophile-initiated thiol-michael reactions advertising niche-independent high-purity cultures social media gastrointestinal stem cells intestinal stem-cell epithelia organoid research pharma research tailed student t-test long-term covalent stabilization niche-inspired culture condition nature+ nature 493 nature 539 nature 459 nature 569 nature 586 nature bonferroni post-hoc analysis biodegradable polymers/block-copolymers université de lausanne extracellular matrix viscoelasticity intestinal stem cell healing ph-sensitive cytosine intestinal organoid development author correspondence tukey post-hoc analysis permissions intestinal crypt formation stem cell transplantation stem-cell colony long-term applicability epithelial organoid derivation tumor organoid-immune springerlink instant access human organoid culture middle stem cell bioengineering enzymatic degradation schemes early development mouse intestinal organoids radiation-crosslinked networks dll-engineering facility culture models dynamic rearrangements mediated

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      headline:Synthetic dynamic hydrogels promote degradation-independent in vitro organogenesis
      description:Epithelial organoids are most efficiently grown from mouse-tumour-derived, reconstituted extracellular matrix hydrogels, whose poorly defined composition, batch-to-batch variability and immunogenicity limit clinical applications. Efforts to replace such ill-defined matrices for organoid culture have largely focused on non-adaptable hydrogels composed of covalently crosslinked hydrophilic macromolecules. However, the excessive forces caused by tissue expansion in such elastic gels severely restrict organoid growth and morphogenesis. Chemical or enzymatic degradation schemes can partially alleviate this problem, but due to their irreversibility, long-term applicability is limited. Here we report a family of synthetic hydrogels that promote extensive organoid morphogenesis through dynamic rearrangements mediated by reversible hydrogen bonding. These tunable matrices are stress relaxing and thus promote efficient crypt budding in intestinal stem-cell epithelia through increased symmetry breaking and Paneth cell formation dependent on yes-associated protein 1. As such, these well-defined gels provide promising versatile matrices for fostering elaborate in vitro morphogenesis. The influence of stress relaxation of the extracellular matrix on the formation of intestinal organoids was investigated. It was shown that a stress-relaxing synthetic matrix promotes crypt budding through increased symmetry breaking and niche cell formation.
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