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organoids, drug, culture, platform, article, organoid, patient, control, cell, tumor, treatment, google, scholar, cancer, device, cells, gemcitabine, microfluidic, cellular, fig, human, chamber, cas, layer, data, treatments, growth, patients, fluidic, automated, analysis, nature, multiplexer, combination, supplementary, research, media, conditions, temporal, dynamic, drugs, screening, system, sensitivity, pdac, chemotherapy, models, individual, channels, size,

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supplementary movie 1 supplementary movie 9 nature portfolio privacy policy advertising reporting summary important tools n-acetyl-l-cysteine [10 mm additional data related nature 466 nature n-acetyl-l-cysteine reprints a-chip stem-cell biotechnology supplementary materials supplementary movies 4–8 supplementary movies 2–3 high-throughput techniques obsolete recombinant human igf-1 author information authors previously published data enables real-time screening supplementary fig single-cell immune dynamics live-cell fluorescence microscopy enables real-time analysis additional genomic analysis thermo fisher scientific open machine-shopped process piece source data resemble traditional plate-based clinical research ranging temporally-delivered gemcitabine + 5-fu compared high-throughput analysis conflict 3d culture-based systems costly patient-derived xenograft patient-derived t-cells24 clinical retrospective data a-chip enables analysis robust high-throughput assay facilitate preclinical research human organ development trimethylchlorosilane-treated silicon mold make silicon-based molds previously published work8 permissions temporally-modified drug treatments high-throughput drug testing traditional plate-based experiments

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      name:Department of Chemistry, The University of Chicago, Chicago, USA
      name:Pritzker School of Molecular Engineering, The University of Chicago, Chicago, USA
      name:Institute for Genomics and Systems Biology, The University of Chicago, Chicago, USA
      name:Pritzker School of Molecular Engineering, The University of Chicago, Chicago, USA
      name:Institute for Genomics and Systems Biology, The University of Chicago, Chicago, USA
      name:Institute for Genomics and Systems Biology, The University of Chicago, Chicago, USA
      name:Institute for Genomics and Systems Biology, The University of Chicago, Chicago, USA
      name:Pritzker School of Molecular Engineering, The University of Chicago, Chicago, USA
      name:Institute for Genomics and Systems Biology, The University of Chicago, Chicago, USA
      name:Department of Pathology, The University of Chicago Medicine, Chicago, USA
      name:Institute for Genomics and Systems Biology, The University of Chicago, Chicago, USA
      name:Committee on Genetics, Genomics and Systems Biology, Departments of Medicine and Human Genetics, The University of Chicago, Chicago, USA
      name:Institute for Genomics and Systems Biology, The University of Chicago, Chicago, USA
      name:Tempus Labs, Chicago, USA
      name:Pritzker School of Molecular Engineering, The University of Chicago, Chicago, USA
      name:Institute for Genomics and Systems Biology, The University of Chicago, Chicago, USA

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