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Keywords {🔍}

ubiquitin, proteasome, chains, degradation, rpn, pubmed, substrate, article, proteins, substrates, google, scholar, cas, protein, klinked, fig, chain, supplementary, receptors, buffer, central, cell, degraded, binding, domain, biol, table, domains, min, receptor, proteasomes, nature, centrifugation, polyubiquitin, data, multiple, wildtype, fluorescence, ubkgfp, column, purified, trishcl, mol, dtt, cells, terminus, ubl, initial, site, function,

Topics {✒️}

nature portfolio privacy policy ubiquitin chain editing research design advertising suc-llvy-amc cleavage activity97 high-resolution cryo-em structure author information authors cdc48-rad23/dsk2 axis contributes social media important development apc/c-mediated multiple monoubiquitylation editing anti-flag immunoaffinity chromatography65 chromosome-coupled ubiquitin-proteasome pathway reprints incubated 50 µm suc‐llvy‐amc n-terminally 6xhis-tagged ubiquitin nature 453 nature 416 nature 532 nature 385 nature 5 mg ml−1 wild-type ubiquitin 25 mg ml−1 wild-type ubiquitin washed ni-nta beads n-terminal 6xhis-tag attached article martinez-fonts original author c-terminal flag-tag65 human anaphase-promoting complex single-molecule kinetic analysis pet duet-derived plasmids pgex-6p-1-derived plasmids substrates kirby martinez-fonts homotypic k11-linked chains34 15 µm ub-35-ub-gfp-tail room-temperature water bath peer review 80 µm e2 ube2s-ubd 60 µm e2 ube2s-ubd core-dependent activity element 4 mm dioxane-free iptg 5 mm dioxane-free iptg 2 mm dioxane-free iptg k48-linked diubiquitin complex ub/ubl-binding uim domain ensure single-turnover conditions ubiquitin-based tagging system 15 mg ml−1 flag peptide

Questions {❓}

• Why do cellular proteins linked to K63-polyubiquitin chains not associate with proteasomes?
• Why does the proteasome have multiple ubiquitin receptors?

Schema {🗺️}

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