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Keywords {🔍}

pip, apical, fig, cysts, lumen, article, cell, formation, ship, arrowheads, cells, basolateral, podxl, google, scholar, cas, mdck, polarity, amis, experiments, polarization, white, control, independent, supplementary, analysis, localization, membrane, vesicles, red, wellsconditionexperiment, nature, raba, inverted, yellow, snx, stained, image, epithelial, quantitation, figure, domain, cyst, expressing, cortical, blue, depletion, biol, images, egfpxphtapp,

Topics {✒️}

nature portfolio privacy policy org/tools/protocols/plko/ rnai-resistant wild-type mcherry-snx9 par3-positive/pip3-depleted zone expanded advertising mutant egfp-2xph-tapp1 unable expressing egfp-2xph-tapp1 [pi class-ii pi3-kinases contribute social media actin-mediated endocytic trafficking class-ii pi3-kinases pik3c2a/ perform high-resolution z-stacks expressing egfp-2xph-tapp1 reprints validated egfp-2xph-tapp1 egfp-2xph-tapp1 r211l p2 [egfp-2xph-tapp1] localization podxl/pip reporter-positive vesicles nature 552 nature 517 nature 499 nature egfp-2xph-tapp1 probe class-ii pi3-kinases24 phosphoinositide binding-deficient mutant class-ii pi3-kinases thermo fisher scientific ship1-egfp phosphatase-dead cells pip3 [egfp-ph-grp1] localization pip-binding defective mutants gfp-tagged pip reporters pip-modifying enzyme expression stably expressing gfp-pik3c2a pip-binding defective mutant apical apkc/cdc42/par3 complex gfp-anx2 relative intensity de novo generation sec14-nodulin domain protein single-mdck cells divide type i-kinases inhibitor de novo polarization rab11a-positive vesicles rearranged multiple lumens/vesicular accumulation semi-automated polarity analysis open lumen stages pi 3-kinase-dependent pathway lumen de novo constitutively rab11a-endosome adjacent podxl-positive vesicular compartments

Questions {❓}

• How is PI(3,4)P2 generated?
• If PI(3,4)P2 is both in recycling endosomes and the apical domain, how can it be an apical determinant?
• Specifically, what controls cell–cell contact remodeling into an AMIS?
• What is the function of apical PI(3,4)P2 in 3D polarization?

Schema {🗺️}

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         headline:The phospholipid PI(3,4)P2 is an apical identity determinant
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            address:
               name:The CRUK Beatson Institute, Glasgow, UK
               type:PostalAddress
            type:Organization
      name:Emma Shanks
      affiliation:
            name:The CRUK Beatson Institute
            address:
               name:The CRUK Beatson Institute, Glasgow, UK
               type:PostalAddress
            type:Organization
      name:Keith E. Mostov
      url:http://orcid.org/0000-0002-8123-6247
      affiliation:
            name:University of California
            address:
               name:Department of Anatomy, University of California, San Francisco, USA
               type:PostalAddress
            type:Organization
            name:University of California
            address:
               name:Department of Biochemistry and Biophysics, University of California, San Francisco, USA
               type:PostalAddress
            type:Organization
      name:David M. Bryant
      url:http://orcid.org/0000-0003-2721-5012
      affiliation:
            name:University of Glasgow
            address:
               name:Institute of Cancer Sciences, University of Glasgow, Glasgow, UK
               type:PostalAddress
            type:Organization
            name:The CRUK Beatson Institute
            address:
               name:The CRUK Beatson Institute, Glasgow, UK
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute of Cancer Sciences, University of Glasgow, Glasgow, UK
      name:The CRUK Beatson Institute, Glasgow, UK
      name:Department of Anatomy, University of California, San Francisco, USA
      name:Department of Biochemistry and Biophysics, University of California, San Francisco, USA
      name:Broad Institute of MIT and Harvard, Cambridge, USA
      name:Institute of Cancer Sciences, University of Glasgow, Glasgow, UK
      name:The CRUK Beatson Institute, Glasgow, UK
      name:Institute of Cancer Sciences, University of Glasgow, Glasgow, UK
      name:The CRUK Beatson Institute, Glasgow, UK
      name:Institute of Cancer Sciences, University of Glasgow, Glasgow, UK
      name:Biochemistry Division, Department of Chemistry, Faculty of Science, Tanta University, Tanta, Egypt
      name:The CRUK Beatson Institute, Glasgow, UK
      name:The CRUK Beatson Institute, Glasgow, UK
      name:Department of Anatomy, University of California, San Francisco, USA
      name:Department of Biochemistry and Biophysics, University of California, San Francisco, USA
      name:Institute of Cancer Sciences, University of Glasgow, Glasgow, UK
      name:The CRUK Beatson Institute, Glasgow, UK

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