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We are analyzing https://www.nature.com/articles/s41467-017-00772-5.

Title:
An HDAC3-PROX1 corepressor module acts on HNF4α to control hepatic triglycerides | Nature Communications
Description:
The histone deacetylase HDAC3 is a critical mediator of hepatic lipid metabolism, and liver-specific deletion of HDAC3 leads to fatty liver. To elucidate the underlying mechanism, here we report a method of cross-linking followed by mass spectrometry to define a high-confidence HDAC3 interactome in vivo that includes the canonical NCoR–HDAC3 complex as well as Prospero-related homeobox 1 protein (PROX1). HDAC3 and PROX1 co-localize extensively on the mouse liver genome, and are co-recruited by hepatocyte nuclear factor 4α (HNF4α). The HDAC3–PROX1 module controls the expression of a gene program regulating lipid homeostasis, and hepatic-specific ablation of either component increases triglyceride content in liver. These findings underscore the importance of specific combinations of transcription factors and coregulators in the fine tuning of organismal metabolism. HDAC3 is a critical mediator of hepatic lipid metabolism and its loss leads to fatty liver. Here, the authors characterize the liver HDAC3 interactome in vivo, provide evidence that HDAC3 interacts with PROX1, and show that HDAC3 and PROX1 control expression of genes regulating lipid homeostasis.
Website Age:
30 years and 10 months (reg. 1994-08-11).

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Keywords {🔍}

hdac, pubmed, prox, liver, article, google, scholar, hnfα, cas, fig, central, expression, gene, hepatic, cell, nuclear, binding, data, lipid, supplementary, control, analysis, transcription, aav, nature, complex, peaks, tbg, sites, transcriptional, interaction, protein, receptor, hdacprox, genes, important, chipseq, metabolism, results, mice, ncor, performed, number, nat, function, proteins, mol, vivo, factors, observed,

Topics {✒️}

nature portfolio err{alpha}/pgc-1{alpha} bioenergetic functions privacy policy temperature-controlled specific-pathogen-free facility rna-seq libraries camp-responsive element-binding protein advertising cross-linking-based sample preparation social media reprints sequence-specific dna-binding factors cholesterol 7-alpha-hydroxylase gene nature 377 nature 473 nature 525 nature 482 nature nf-κb protein p65 repress trans-activation induced multimodal learning-based prediction cholesterol 7alpha-hydroxylase gene gov/geo/query/acc rnase-free dnase set central nervous system 100 µg ml−1 penicillin/streptomycin wilcoxon–mann–whitney test mammalian ftz-f1 homologue anti-aging interventions ahmed prospero-related homeodomain protein wd40-repeat protein linked anti-flag m2 resin aav-based knockdown vectors ip chromatin-protein complexes hnf4α fl/fl mice high fat diet anti-flag m2 hrp describe neat chip-ms mouse lens-fibre elongation hdac3 fl/fl animals chrebp-dependent hepatic steatosis n-cor corepressors intensity-based absolute quantification thermo fisher scientific scientific advisory boards cross-linking methods developed cross-linking isolated nuclei developed neat chip-ms wild-type mice infected hepatic gluconeogenesis amy hdac3–prox1 module controls

Questions {❓}

Schema {🗺️}

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      headline:An HDAC3-PROX1 corepressor module acts on HNF4α to control hepatic triglycerides
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      affiliation:
            name:Perelman School of Medicine at the University of Pennsylvania
            address:
               name:Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
            name:Perelman School of Medicine at the University of Pennsylvania
            address:
               name:Divison of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
      name:Zhenghui Li
      affiliation:
            name:Perelman School of Medicine at the University of Pennsylvania
            address:
               name:Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
            name:Perelman School of Medicine at the University of Pennsylvania
            address:
               name:Divison of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
      name:Benjamin A. Garcia
      affiliation:
            name:Perelman School of Medicine at the University of Pennsylvania
            address:
               name:Department of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
      name:Mitchell A. Lazar
      affiliation:
            name:Perelman School of Medicine at the University of Pennsylvania
            address:
               name:Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
            name:Perelman School of Medicine at the University of Pennsylvania
            address:
               name:Divison of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Divison of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Divison of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Department of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Divison of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Department of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Divison of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Divison of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Department of Biochemistry and Biophysics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA
      name:Divison of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA

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