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We are analyzing https://www.nature.com/articles/s41418-018-0236-y.

Title:
KLF5 and MYC modulated LINC00346 contributes to gastric cancer progression through acting as a competing endogeous RNA and indicates poor outcome | Cell Death & Differentiation
Description:
It was found in this study that long intergenic non-protein coding RNA 346 (LINC00346) was an lncRNA aberrantly expressed in gastric cancer (GC) based on multiple Gene Expression Omnibus (GEO) databases of GC cohorts. The LINC00346 gene was recurrently amplified and upregulated in GC, and its expression was positively correlated with poor pathologic stage, large tumor size, and poor prognosis. In addition, the oncogenic transcription factors KLF5 and MYC could bind to the LINC00346 promoter and enhance its expression. Gene Set Enrichment Analysis (GSEA) in the GEO datasets revealed that cell cycle and focal adhesion genes were enriched in patients with high LINC00346 expression. In vitro and in vivo assays of LINC00346 alterations revealed a complex integrated phenotype affecting cell growth, migration and invasion. Strikingly, high-throughput sequencing analysis after LINC00346 alterations highlighted alterations in cell cycle and focal adhesion pathways in GC cells. Mechanistically, argonaute 2 (Ago2) was recruited by LINC00346, which functioned as a molecular sponge for miR-34a-5p by antagonizing its ability to repress CD44, NOTCH1, and AXL protein translation. Taken together, our findings support a model in which the KLF5, MYC/LINC00346/miR-34a-5p cross-talk served as critical effectors in GC tumorigenesis and progression, suggesting a new therapeutic direction in the treatment of GC.
Website Age:
30 years and 10 months (reg. 1994-08-11).

Matching Content Categories {📚}

  • Education
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Custom-built

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🌠 Phenomenal Traffic: 5M - 10M visitors per month


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$63,100 per month
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Keywords {🔍}

linc, expression, fig, cell, cells, supplementary, cancer, notch, pubmed, axl, article, gene, klf, analysis, rna, gastric, myc, mirap, google, scholar, data, assays, gse, tissues, cas, patients, proliferation, table, overexpression, performed, invasion, found, mira, nature, tumor, enrichment, migration, lncrnas, showed, wang, luciferase, genes, noncoding, metastasis, long, lncrna, high, protein, vector, scnas,

Topics {✒️}

nature portfolio myc/linc00346/mir-34a-5p cross-talk served permissions reprints privacy policy regional lymph-node metastasis hidden rna language tincr/stau1/cdkn2b signaling axis advertising nature 2014 nature clinical research center mir-34a-5p response element social media mir-34a-5p targeting sites linc00346 sponged mir-34a-5p linc00346-mediated cell proliferation mir-34a-5p-treated cells human research open platform author information authors mir-34a-5p target gene probes targeting mir-34a-5p yong-qian shu lineage-survival oncogenes klf5 mir-16-5p/ccnd2 axis supplementary table 3 supplementary table 4 supplementary table 5 supplementary table 6 supplementary table 7 supplementary table 9 supplementary table 10 supplementary table 1 supplementary table 2 mir-34a-5p reduced inhibitor mir-34a reduced somatic super-enhancer duplications author correspondence full access mir-34a-5p mimic research web server issue mir-34a-5p decreased endogenous mir-34a-5p somatic copy-number alteration henceforth named linc00346-mut1 somatic copy-number alterations high-throughput sequencing analysis linc00346–induced cell proliferation wilcoxon signed-rank test

Questions {❓}

  • A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language?
  • Proteolysis of TAM receptors in autoimmune diseases and cancer: what does it say to us?

Schema {🗺️}

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Libraries {📚}

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Mail Servers:

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