PMC . NCBI . NLM . NIH . GOV {
}
We are analyzing https://pmc.ncbi.nlm.nih.gov/articles/PMC5216816/.
Title:
CRISPR-dCas9 mediated TET1 targeting for selective DNA demethylation at BRCA1 promoter - PMC
Description:
DNA hypermethylation at the promoter of tumour-suppressor genes is tightly correlated with their transcriptional repression and recognized as the hallmark of majority of cancers. Epigenetic silencing of tumour suppressor genes impairs their cellular ...
Website Age:
27 years and 8 months (reg. 1997-10-02).
Title:
CRISPR-dCas9 mediated TET1 targeting for selective DNA demethylation at BRCA1 promoter - PMC
Description:
DNA hypermethylation at the promoter of tumour-suppressor genes is tightly correlated with their transcriptional repression and recognized as the hallmark of majority of cancers. Epigenetic silencing of tumour suppressor genes impairs their cellular ...
Website Age:
27 years and 8 months (reg. 1997-10-02).
Matching Content Categories {📚}
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Custom-built
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🌠 Phenomenal Traffic: 5M - 10M visitors per month
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Keywords {🔍}
doi, brca, sgrna, demethylation, cells, figure, cpg, dna, sites, tdei, pubmed, google, scholar, promoter, methylation, gene, tet, cell, expression, fusion, binding, site, cancer, target, pmc, proteins, sequence, observed, sgrnas, protein, tdeii, article, epigenetic, hela, hmc, free, targeted, sequenced, cpgs, transfection, mcf, region, tetcd, pcr, dcas, transcription, combination, specific, primer, increase,
Topics {✒️}
hif-1α-vegf mediated angiogenesis paav_ef1α_tet1cd-dcas9-nls-2a-gfp_wpre_hghpa tris-buffered-saline-tween buffer crispr/cas9-tet1 mediated demethylation 5-diphenyl-2h-tetrazolium bromide engineered crispr-cas9 complex ten-eleven translocation dioxygenase crispr/dcas9-tet1 target sites pmc beta search quantitative real-time pcr high-throughput functional genomics short palindromic repeat-crispr designed single-guide rnas tet1cd-dcas9-mediated brca1 overexpression li-cor odyssey scanner create de-novo methylation adenovirus-mediated brca1 overexpression crispr-tet1 targeted sites tet1-dcas9 fusion protein supplementary materials oncotarget-07-46545-s001 mammalian germ-cell development silenced/downregulated gene expression single-guide rna supplementary data oncotarget-07-46545-s001 genome-scale transcriptional activation tet1cd-dcas9 fusion protein tet1cd-dcas9 fusion proteins dcas9-tet1cd fusion proteins ten-eleven translocation tet1cd-dcas9-mediated increase full nucleotide sequence crispr-cas systems tumour-suppressor genes tumour suppressor genes tde-ii enables demethylation full length sequence tde-ii binding site bradford assay kit dmem/f-12 supplemented rneasy mini kit crispr-cas9 system crispr targeted tet1 tet-mediated formation advancing translational sciences translational sciences award tet1 mediated targeting final fusion protein pyromark pcr kit line-1 repeat sequence histone covalent modifications
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