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Title:
Glutaminolysis and Transferrin Regulate Ferroptosis - PMC
Description:
Ferroptosis has emerged as a new form of regulated necrosis that is implicated in various human diseases. However, the mechanisms of ferroptosis are not well defined. This study reports the discovery of multiple molecular components of ferroptosis ...
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Keywords {🔍}
cell, death, doi, figure, ferroptosis, necrosis, mefs, amino, transferrin, google, scholar, pubmed, starvation, activity, serum, serumdependent, treated, pmc, glutaminolysis, acid, cells, fbs, article, hrs, cellular, cystine, free, measured, lgln, glutamine, subsequently, protein, induce, inhibitor, induced, injury, glutathione, killing, gls, apoptosis, process, viability, difbs, acids, essential, rip, compound, levels, nature, required,
Topics {✒️}
l-g-glutamyl-p-nitroanilide amino acid/serum-free medium iron-free bovine apo-transferrin ischemia/reperfusion-induced organ damage pan-caspase inhibitor zvad-fmk amino acid/serum-double starvation trigger serum-dependent necrosis amino acid-free medium maldi-tof-ms/ms amino acid-free conditions inhibited serum-dependent necrosis bax/bak-double knockout seemingly counter-intuitive observations confocal time-lapse imaging pmc beta search recombinant human holo-transferrin inhibit serum-dependent necrosis inhibit serum-induced necrosis block serum-dependent necrosis gamma-glutamylcysteine synthetase deficiency rip3-dependent necrosis pathway gamma-glutamyl cysteine synthetase full experimental procedures genotype-selective antitumor agents serum-induced cell death stable free radical ischemia-reperfusion heart injury reverse phase-c18 hplc reversed phase xdb-c18 ngamma-aryl glutamine analogues tumor necrosis factor-α downstream necrotic response blocked erastin-induced ferroptosis supplementary materials included aldose reductase pathway rat kidney-type glutaminase gamma-glutamylcysteine synthetase gene cell type-dependent manner reduce ischemia-reperfusion injuries rip3-indepednent cell death death-inducing activity ranged ras-erk signaling mechanisms controlling iron-loading growth/survival factors function glutamine dose-dependent manner inhibit erastin-induced ferroptosis unpaired student t-test mitochondrial oxidative phosphorylation nutrient/growth factor deprivation cystine/glutamate antiporter slc7a11
Questions {❓}
- Does serum-dependent necrosis require deprivation of all amino acids or certain specific amino acid(s)?
- How do transferrin, cellular glutaminolysis, glutathione synthesis, and cellular ROS generation process communicate with each other to lead to cell death?
- In light of our finding that ferroptosis requires glutaminolysis, a metabolic pathway highly active in cancer tissues and essential for cancer growth, can ferroptosis be preferentially triggered in cancer cells as a therapeutic option?
- Under what biological conditions can ferroptosis, a necrotic process induced experimentally by cystine starvation or blockage of cystine uptake, occur?
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