
PMC . NCBI . NLM . NIH . GOV {
}
Title:
Phosphorylation of human progesterone receptors at serine-294 by mitogen-activated protein kinase signals their degradation by the 26S proteasome - PMC
Description:
Ligand-dependent down-regulation that leads to rapid and extensive loss of protein is characteristic of several nuclear steroid receptors, including human progesterone receptors (PRs). In breast cancer cells, >95% of PRs are degraded 6 h after the ...
Website Age:
27 years and 9 months (reg. 1997-10-02).
Matching Content Categories {π}
- Science
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Custom-built
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What is the average monthly size of pmc.ncbi.nlm.nih.gov audience?
π Phenomenal Traffic: 5M - 10M visitors per month
Based on our best estimate, this website will receive around 5,000,025 visitors per month in the current month.
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Keywords {π}
doi, cells, google, scholar, pubmed, mapk, prs, phosphorylation, downregulation, protein, degradation, fig, prb, proteasome, receptors, mutant, mapks, serine, treated, receptor, inhibitor, antibodies, presence, pmc, levels, immunoblotting, wildtype, biol, liganddependent, cancer, lactacystin, measured, human, expression, tdyb, horwitz, article, ligand, cell, ref, stably, activation, lysates, ubiquitin, breast, specific, sites, ligandindependent, pathway, destruction,
Topics {βοΈ}
n-acetyl-leu-leu-nle-cho n-acetyl-leu-leu-met-cho mitogen-activated protein kinases epitope-flag-tagged wild-type pr high-molecular-weight ubiquitinated forms growth factor responsiveness growth factor addition serum-starved t47d-yb cells ha-tagged ubiquitin construct advanced-stage breast cancers hemagglutinin geldanamycin flag-tagged wild-type flag-tagged pr-bs egf-treated t47d-yb cells chaperone-assembly factor monoclonal pr-negative t47d pmc beta search s294+344/345a pr-bs serineβproline consensus sites induce ligand-dependent receptor flag-specific antibodies demonstrated demonstrate ubiquitin-conjugated pr wild-type pr-b ha-tagged ubiquitin pr-specific monoclonal antibodies ga-treated cells fail s294a mutant pr-bs serum-free medium trap pr-ubiquitin conjugates unliganded immature pr-bs ubiquitin-dependent proteasomal mechanism ligand-induced receptor phosphorylation phosphorylated active kinase progestin-activated mapks cyclin-dependent kinase 2 advanced breast cancer human progesterone receptors s294+s344/345a pr clear activated receptors large molecular size egf [30 ng/ml ligand-dependent pr degradation ubiquitin-conjugating enzymes s344a/s345a mutant pr ligand-independent pr degradation receptor expression vectors similar affinity gel t47d-yb cells pretreated ha-specific antibodies
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