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         headline:Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum
         description:Phaeodactylum tricornutum (Pt) is a critical microbial cell factory to produce a wide spectrum of marketable products including recombinant biopharmaceutical N-glycoproteins. N-glycosylation modification of proteins is important for their activity, stability, and half-life, especially some special modifications, such as fucose-modification by fucosyltransferase (FucT). Three PtFucTs were annotated in the genome of P. tricornutum, PtFucT1 was located on the medial/trans-Golgi apparatus and PtFucT2-3 in the plastid stroma. Algal growth, biomass and photosynthesis efficiency were significantly inhibited in a knockout mutant of PtFucT1 (PtFucT1-KO). PtFucT1 played a role in non-core fucose modification of N-glycans. The knockout of PtFucT1 might affect the activity of PtGnTI in the complex and change the complex N-glycan to mannose type N-glycan. The study provided critical information for understanding the mechanism of protein N-glycosylation modification and using microalgae as an alternative ecofriendly cell factory to produce biopharmaceuticals.
         datePublished:2023-01-07T00:00:00Z
         dateModified:2023-01-07T00:00:00Z
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            Glycoprotein
            Fucosyltransferase
            N-glycan
            Microbial cell factory
            Applied Microbiology
            Biotechnology
            Microbiology
            Microbial Genetics and Genomics
            Enzymology
            Genetic Engineering
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      headline:Golgi fucosyltransferase 1 reveals its important role in α-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum
      description:Phaeodactylum tricornutum (Pt) is a critical microbial cell factory to produce a wide spectrum of marketable products including recombinant biopharmaceutical N-glycoproteins. N-glycosylation modification of proteins is important for their activity, stability, and half-life, especially some special modifications, such as fucose-modification by fucosyltransferase (FucT). Three PtFucTs were annotated in the genome of P. tricornutum, PtFucT1 was located on the medial/trans-Golgi apparatus and PtFucT2-3 in the plastid stroma. Algal growth, biomass and photosynthesis efficiency were significantly inhibited in a knockout mutant of PtFucT1 (PtFucT1-KO). PtFucT1 played a role in non-core fucose modification of N-glycans. The knockout of PtFucT1 might affect the activity of PtGnTI in the complex and change the complex N-glycan to mannose type N-glycan. The study provided critical information for understanding the mechanism of protein N-glycosylation modification and using microalgae as an alternative ecofriendly cell factory to produce biopharmaceuticals.
      datePublished:2023-01-07T00:00:00Z
      dateModified:2023-01-07T00:00:00Z
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      pageEnd:18
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          Phaeodactylum tricornutum
         Glycoprotein
         Fucosyltransferase
         N-glycan
         Microbial cell factory
         Applied Microbiology
         Biotechnology
         Microbiology
         Microbial Genetics and Genomics
         Enzymology
         Genetic Engineering
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      name:Xiaojuan Liu
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      name:Guangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou University, Shantou, China
      name:Yantai Academy of Agricultural Sciences, Yantai, China
      name:Guangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou University, Shantou, China
      name:Guangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou University, Shantou, China
      name:Guangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou University, Shantou, China
      name:Guangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou University, Shantou, China
      name:Guangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou University, Shantou, China
      name:Guangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou University, Shantou, China
      name:Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China
      name:Guangdong Provincial Key Laboratory of Marine Biotechnology, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Institute of Marine Sciences, STU-UNIVPM Joint Algal Research Center, College of Sciences, Shantou University, Shantou, China

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• What's the revenue for http://scholar.google.com/scholar_lookup?&title=Multiplex%20peptide%20stable%20isotope%20dimethyl%20labeling%20for%20quantitative%20proteomics&journal=Nat%20Protoc&doi=10.1038%2Fnprot.2009.21&volume=4&pages=484-494&publication_year=2009&author=Boersema%2CPJ&author=Raijmakers%2CR&author=Lemeer%2CS&author=Mohammed%2CS&author=Heck%2CAJR?
• Learn how profitable https://doi.org/10.1021%2Facs.analchem.6b01388 is on a monthly basis
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• How much income does http://scholar.google.com/scholar_lookup?&title=Site-specific%20fucosylation%20analysis%20identifying%20glycoproteins%20associated%20with%20aggressive%20prostate%20cancer%20cell%20lines%20using%20tandem%20affinity%20enrichment%20of%20intact%20glycopeptides%20followed%20by%20mass%20spectrometry&journal=Ann%20Chem&volume=176&pages=139-148&publication_year=2017&author=Zhou%2CJ&author=Yang%2CW&author=Hu%2CY&author=Hoti%2CN&author=Liu%2CY&author=Shah%2CP&author=Sun%2CS&author=Clark%2CD&author=Thomas%2CS&author=Zhang%2CH have?
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• What are the total earnings of https://doi.org/10.3389%2Ffgene.2019.00372?
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• What's the profit of https://doi.org/10.1093%2Fnar%2Fgky995?
• How much cash flow does http://scholar.google.com/scholar_lookup?&title=The%20Pfam%20protein%20families%20database%20in%202019&journal=Nucleic%20Acids%20Res&doi=10.1093%2Fnar%2Fgky995&volume=47&pages=D427-D432&publication_year=2019&author=El-Gebali%2CS&author=Mistry%2CJ&author=Bateman%2CA&author=Eddy%2CSR&author=Luciani%2CA&author=Potter%2CSC&author=Qureshi%2CM&author=Richardson%2CLJ&author=Salazar%2CGA&author=Smart%2CA&author=Sonnhammer%2CELL&author=Hirsh%2CL&author=Paladin%2CL&author=Piovesan%2CD&author=Tosatto%2CSCE&author=Finn%2CRD have monthly?
• Revenue of https://doi.org/10.1093%2Fglycob%2Fcwr056
• See how much http://scholar.google.com/scholar_lookup?&title=Distantly-related%20plant%20and%20nematode%20core%20%CE%B11%2C3-fucosyltransferases%20display%20similar%20trends%20in%20structure-function%20relationships&journal=Glycobiology&doi=10.1093%2Fglycob%2Fcwr056&volume=21&pages=1401-1415&publication_year=2012&author=Both%2CP&author=Sobczak%2CL&author=Breton%2CC&author=Hann%2CS&author=N%C3%B6bauer%2CK&author=Paschinger%2CK&author=Kozmon%2CS&author=Mucha%2CJ&author=Wilson%2CIBH makes per month
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• Financial intake of https://doi.org/10.1038%2Fs41598-019-42617-9
• How much money does http://scholar.google.com/scholar_lookup?&title=Targeting%20mutations%20to%20the%20plastidial%20psbA%20gene%20of%20Chlamydomonas%20reinhardtii%20without%20direct%20positive%20selection&journal=Sci%20Rep&doi=10.1038%2Fs41598-019-42617-9&volume=9&publication_year=2019&author=Shmidt%2CV&author=Kaftan%2CD&author=Scherz%2CA&author=Danon%2CA make?
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