We are analyzing https://link.springer.com/protocol/10.1385/1-59259-828-5:099.

Title:
Site-Specific Analysis of Histone Methylation and Acetylation | SpringerLink
Description:
Covalent modifications on the nucleosomal histones are essential in chromatin regulation and gene expression. Patterns of histone modifications may be somatically maintained and can thereby maintain locus-specific repression/activity in defined lineages or throughout...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Site-Specific Analysis of Histone Methylation and Acetylation
      pageEnd:120
      pageStart:99
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-59259-828-1.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Epigenetics Protocols
         isbn:
            978-1-59259-828-1
            978-1-58829-336-7
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:David Umlauf
            affiliation:
                  name:CNRS UMR-5535 and University of Montpellier II
                  address:
                     name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yuji Goto
            affiliation:
                  name:CNRS UMR-5535 and University of Montpellier II
                  address:
                     name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Robert Feil
            affiliation:
                  name:CNRS UMR-5535 and University of Montpellier II
                  address:
                     name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Chromatin immunoprecipitation, native chromatin, histone methylation, histone acetylation, quantitative PCR, allele-specific PCR
      description:Covalent modifications on the nucleosomal histones are essential in chromatin regulation and gene expression. Patterns of histone modifications may be somatically maintained and can thereby maintain locus-specific repression/activity in defined lineages or throughout development. During recent years, histone acetylation and methylation have emerged as key players in the repression or activation of genes and chromosomal domains. Histone methylation and acetylation patterns (and other histone modifications) can be analyzed by chromatin immunoprecipitation (ChIP). This chapter describes how ChIP can be performed on native chromatin prepared from cells and tissues, in order to analyze histone methylation and acetylation at specific sites in the genome. We also present different PCR-based assays that can be applied to analyze loci of interest in immunoprecipitated chromatin fractions.
      datePublished:2004
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Book:
      name:Epigenetics Protocols
      isbn:
         978-1-59259-828-1
         978-1-58829-336-7
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      name:Humana Press
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      name:CNRS UMR-5535 and University of Montpellier II
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         name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
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      name:CNRS UMR-5535 and University of Montpellier II
      address:
         name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
         type:PostalAddress
      name:CNRS UMR-5535 and University of Montpellier II
      address:
         name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
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      name:David Umlauf
      affiliation:
            name:CNRS UMR-5535 and University of Montpellier II
            address:
               name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
               type:PostalAddress
            type:Organization
      name:Yuji Goto
      affiliation:
            name:CNRS UMR-5535 and University of Montpellier II
            address:
               name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
               type:PostalAddress
            type:Organization
      name:Robert Feil
      affiliation:
            name:CNRS UMR-5535 and University of Montpellier II
            address:
               name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
      name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
      name:Institute for Molecular Genetics, CNRS UMR-5535 and University of Montpellier II, Montpellier, France
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