We are analyzing https://link.springer.com/protocol/10.1385/1-59259-793-9:263.

Title:
Transfection of the Human Malaria Parasite Plasmodium falciparum | SpringerLink
Description:
Methods to transiently and stably transfect blood stages of the human malaria parasite Plasmodium falciparum have been developed and adapted for gene-knockout, allelic replacement, and transgene expression in this organism. These methods are detailed in this chapter,...
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Keywords {🔍}

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Topics {✒️}

parasite genomics protocols privacy choices/manage cookies transgenic rodent parasites plasmodium falciparum chromosomes protocol cite humana press journal finder publish european economic area tim-wolf gilberger physiological shear stress nucleic acids res puromycin-n-acetyltransferase pgh1 modulates sensitivity eliza hall institute conditions privacy policy double crossover recombination independent selectable markers protocol crabb accepting optional cookies stable transgene expression medical research main content log plasmodium falciparum gene targeting journal publish privacy policy personal data check access ethics access gene-knockout australia brendan melville rights permissions reprints books a transgene expression stable transfection optional cookies manage preferences plasmid vectors efficient segregation protein trafficking chapter log data protection essential cookies cookies skip subscription content similar content nonhomologous recombination selectable marker nature 403

Schema {🗺️}

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      headline:Transfection of the Human Malaria Parasite Plasmodium falciparum
      pageEnd:276
      pageStart:263
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      keywords:Allelic replacement, DHFR, GFP, knockout, malaria, negative selection, Plasmodium falciparum, Rep20, selectable marker, thymidine kinase, transfection, transgene expression
      description:Methods to transiently and stably transfect blood stages of the human malaria parasite Plasmodium falciparum have been developed and adapted for gene-knockout, allelic replacement, and transgene expression in this organism. These methods are detailed in this chapter, as are approaches used to monitor transfectants during the selection process. The different plasmid vectors that are currently used for gene targeting and transgene expression (including green fluorescent protein expression) are also described.
      datePublished:2004
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