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Title:
Assays for Transcriptional Activity Based on the Luciferase Reporter Gene | SpringerLink
Description:
Reporter genes provide easy and efficient methods for the indirect measurement of relative rates of transcription. Utilizing common DNA cloning methods, a putative regulatory region can be coupled to the coding sequence of a reporter gene such that expression of the...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Assays for Transcriptional Activity Based on the Luciferase Reporter Gene
      pageEnd:174
      pageStart:165
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-59259-686-7.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Transcription Factor Protocols
         isbn:
            978-1-59259-686-7
            978-0-89603-573-7
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:S. Roy Himes
            affiliation:
                  name:University of Queensland
                  address:
                     name:Department of Microbiology, University of Queensland, Brisbane, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:M. Frances Shannon
            affiliation:
                  name:Australian National University
                  address:
                     name:Department of Biochemistry John Curtin School of Medical Research, Australian National University, Canberra, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Luciferase Activity, Potassium Phosphate Buffer, Transfection Efficiency, Reporter Construct, Reporter System
      description:Reporter genes provide easy and efficient methods for the indirect measurement of relative rates of transcription. Utilizing common DNA cloning methods, a putative regulatory region can be coupled to the coding sequence of a reporter gene such that expression of the reporter protein product varies according to the regulatory potential of the DNA tested. The assays for reporter enzymes have the advantage of high sensitivity with low background, and, although an indirect measure, the amount of protein product is usually directly proportional to the level of transcriptional activation. Alternatives to reporter gene assays such as the direct measurement of the level of specific mRNAs for the endogenous gene can be influenced by RNA stability changes as well as transcription rates in response to stimulation. Assays for mRNA are also more labor intensive and difficult to quantify.
      datePublished:2000
      isAccessibleForFree:
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Book:
      name:Transcription Factor Protocols
      isbn:
         978-1-59259-686-7
         978-0-89603-573-7
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Queensland
      address:
         name:Department of Microbiology, University of Queensland, Brisbane, Australia
         type:PostalAddress
      name:Australian National University
      address:
         name:Department of Biochemistry John Curtin School of Medical Research, Australian National University, Canberra, Australia
         type:PostalAddress
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Person:
      name:S. Roy Himes
      affiliation:
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            address:
               name:Department of Microbiology, University of Queensland, Brisbane, Australia
               type:PostalAddress
            type:Organization
      name:M. Frances Shannon
      affiliation:
            name:Australian National University
            address:
               name:Department of Biochemistry John Curtin School of Medical Research, Australian National University, Canberra, Australia
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Microbiology, University of Queensland, Brisbane, Australia
      name:Department of Biochemistry John Curtin School of Medical Research, Australian National University, Canberra, Australia
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