We are analyzing https://link.springer.com/protocol/10.1385/1-59259-141-8:137.

Title:
Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun | SpringerLink
Description:
Gene gun technology provides a useful means for direct transfer of DNA or RNA constructs that can result in transgenic protein expression from gene expression vectors (1–8). The system has been applied to a broad spectrum of experimental studies on transgenic...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

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      headline:Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun
      pageEnd:147
      pageStart:137
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-59259-141-1.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Gene Therapy Protocols
         isbn:
            978-1-59259-141-1
            978-0-89603-723-6
         type:Book
      publisher:
         name:Springer New York
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      author:
            name:Chia-Feng Kuo
            affiliation:
                  name:Shih-Chen University
                  address:
                     name:Department of Foods & Nutrition, Shih-Chen University, Taipei, Taiwan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jeng Hwan Wang
            affiliation:
                  name:Shih-Chen University
                  address:
                     name:Department of Foods & Nutrition, Shih-Chen University, Taipei, Taiwan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ning-Sun Yang
            affiliation:
                  name:Shih-Chen University
                  address:
                     name:Department of Foods & Nutrition, Shih-Chen University, Taipei, Taiwan
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Gold Particle, Skin Tissue, Gene Therapy Approach, Potassium Phosphate Monobasic, Gold Bead
      description:Gene gun technology provides a useful means for direct transfer of DNA or RNA constructs that can result in transgenic protein expression from gene expression vectors (1–8). The system has been applied to a broad spectrum of experimental studies on transgenic research, gene therapy approaches, and genetic vaccinations (see Note 1). The technology was first reported by Yang et al. in 1990 (1) for in vivo and in vitro gene transfer into mammalian somatic tissues and was later extended to various ex vivo gene transfer systems, including excised tissue explants or clumps, organic tissues placed in culture vessels, and their derived primary cultures (9; see Note 2).
      datePublished:2002
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               type:PostalAddress
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      name:Jeng Hwan Wang
      affiliation:
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               type:PostalAddress
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      name:Ning-Sun Yang
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            address:
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PostalAddress:
      name:Department of Foods & Nutrition, Shih-Chen University, Taipei, Taiwan
      name:Department of Foods & Nutrition, Shih-Chen University, Taipei, Taiwan
      name:Department of Foods & Nutrition, Shih-Chen University, Taipei, Taiwan
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