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Title:
Isolation and Culture of Murine Primary Chondrocytes | SpringerLink
Description:
To identify factors that are necessary and sufficient for chondrocyte hypertrophic differentiation and cartilage matrix mineralization, primary chondrocyte culture models have been developed. Here we describe the isolation, short-term and long-term culture, and...
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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Isolation and Culture of Murine Primary Chondrocytes
      pageEnd:277
      pageStart:267
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-989-5.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Skeletal Development and Repair
         isbn:
            978-1-62703-989-5
            978-1-62703-988-8
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Anthony J. Mirando
            affiliation:
                  name:University of Rochester Medical Center
                  address:
                     name:University of Rochester Medical Center, Rochester, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yufeng Dong
            affiliation:
                  name:University of Rochester Medical Center
                  address:
                     name:University of Rochester Medical Center, Rochester, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jinsil Kim
            affiliation:
                  name:University of Rochester Medical Center
                  address:
                     name:University of Rochester Medical Center, Rochester, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Matthew J. Hilton
            affiliation:
                  name:University of Rochester Medical Center
                  address:
                     name:University of Rochester Medical Center, Rochester, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Cartilage, Chondrocyte, Hypertrophy, Mineralization, Cell culture
      description:To identify factors that are necessary and sufficient for chondrocyte hypertrophic differentiation and cartilage matrix mineralization, primary chondrocyte culture models have been developed. Here we describe the isolation, short-term and long-term culture, and analysis of primary costal chondrocytes from the mouse. Briefly, sternae and rib cages from neonatal pups are dissected, and chondrocytes are isolated via enzymatic digestions. Chondrocytes are then plated at high density and cultured in the presence of ascorbic acid and beta-glycerophosphate as well as various recombinant proteins to promote or inhibit hypertrophic differentiation. We also describe the use of adenoviruses to recombine floxed alleles and over-express genes within these cultures. Finally, we detail methods for alkaline phosphatase and alizarin red staining that are used to visualize chondrocyte maturation and cartilage matrix mineralization.
      datePublished:2014
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Book:
      name:Skeletal Development and Repair
      isbn:
         978-1-62703-989-5
         978-1-62703-988-8
Organization:
      name:Humana Press
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         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
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      name:University of Rochester Medical Center
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      affiliation:
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            address:
               name:University of Rochester Medical Center, Rochester, USA
               type:PostalAddress
            type:Organization
      name:Yufeng Dong
      affiliation:
            name:University of Rochester Medical Center
            address:
               name:University of Rochester Medical Center, Rochester, USA
               type:PostalAddress
            type:Organization
      name:Jinsil Kim
      affiliation:
            name:University of Rochester Medical Center
            address:
               name:University of Rochester Medical Center, Rochester, USA
               type:PostalAddress
            type:Organization
      name:Matthew J. Hilton
      affiliation:
            name:University of Rochester Medical Center
            address:
               name:University of Rochester Medical Center, Rochester, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:University of Rochester Medical Center, Rochester, USA
      name:University of Rochester Medical Center, Rochester, USA
      name:University of Rochester Medical Center, Rochester, USA
      name:University of Rochester Medical Center, Rochester, USA
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