We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-694-8_10.

Title:
Small RNA Library Construction from Minute Biological Samples | SpringerLink
Description:
Increasingly, the discovery and characterization of small regulatory RNAs from a variety of organisms have all required deep-sequencing methodologies. However, the crux to successful deep-sequencing analysis depends upon optimal construction of a cDNA library...
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Technology & Computing

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We can't see how the site brings in money.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com has a revenue plan, but it's either invisible or we haven't found it.

Keywords {🔍}

pubmed, small, article, scholar, google, rnas, cas, rna, methods, central, drosophila, sequencing, protocol, library, lau, analysis, piwiinteracting, micrornas, genes, biol, privacy, cookies, content, information, publish, minute, biology, mol, siomi, research, search, construction, samples, igloi, regulatory, cdna, endogenous, pirnas, access, chapter, bartel, science, cell, dev, download, springer, usd, personal, data, log,

Topics {✒️}

month download article/chapter xenopus piwi-interacting rnas dicer-dependent small rnas required deep-sequencing methodologies high-throughput sequencing platform piwi-interacting rnas gung-wei chirn & nelson small rna-seq methods small rna profiling cdna library sequencing privacy choices/manage cookies cdna library compatible device instant download small endogenous rnas enriched follicle cells small rna sequencing small rna preparations small rna cloning drosophila piwi proteins small rnas isolated small regulatory rnas regulatory small rnas small expressed rnas plant small rnas author information authors gung-wei chirn deep sequencing technology single cell analysis drosophila rna methyltransferase searle scholar large-scale sequencing european economic area antisense oligonucleotide depletion polyacrylamide gel system probable regulatory roles mediates 2′-o-methylation babiarz je substantially expanded set orr-weaver tl van den engh structural rna fragments minute biological samples journal finder publish conditions privacy policy ruby jg previously annotated genes differentially expressed genes successful library accepting optional cookies modifies germline pirnas

Schema {🗺️}

ScholarlyArticle:
      headline:Small RNA Library Construction from Minute Biological Samples
      pageEnd:136
      pageStart:123
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-694-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:PIWI-Interacting RNAs
         isbn:
            978-1-62703-694-8
            978-1-62703-693-1
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Jessica A. Matts
            affiliation:
                  name:Brandeis University
                  address:
                     name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Yuliya Sytnikova
            affiliation:
                  name:Brandeis University
                  address:
                     name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Gung-wei Chirn
            affiliation:
                  name:Brandeis University
                  address:
                     name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Gabor L. Igloi
            affiliation:
                  name:University of Freiburg
                  address:
                     name:Institute of Biology, University of Freiburg, Freiburg, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Nelson C. Lau
            affiliation:
                  name:Brandeis University
                  address:
                     name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Small RNAs, Illumina deep-sequencing, Library construction
      description:Increasingly, the discovery and characterization of small regulatory RNAs from a variety of organisms have all required deep-sequencing methodologies. However, the crux to successful deep-sequencing analysis depends upon optimal construction of a cDNA library compatible for the high-throughput sequencing platform. Challenges to small RNA library constructions arise when dealing with minute tissue samples because certain structural RNA fragments can dominate and mask the desired characterization of regulatory small RNAs like microRNAs (miRNAs), endogenous small interfering RNAs (endo-siRNAs), and Piwi-interacting RNAs (piRNAs). Here, we describe methods that improve the chances of constructing a successful library from small RNAs isolated from minute tissues such as enriched follicle cells from the Drosophila ovarium. Because the ribosomal RNA (rRNA) fragments are frequently the major contaminants in small RNA preparations from minute amounts of tissue, we demonstrate the utility of antisense oligonucleotide depletion and an acryloylaminophenylboronic acid (APB) polyacrylamide gel system for separating the abundant 2S rRNA in Drosophila from endo-siRNAs and piRNAs. Finally, our methodology generates libraries amenable to multiplex sequencing on the Illumina Hi-Seq platform.
      datePublished:2014
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:PIWI-Interacting RNAs
      isbn:
         978-1-62703-694-8
         978-1-62703-693-1
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Brandeis University
      address:
         name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
         type:PostalAddress
      name:Brandeis University
      address:
         name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
         type:PostalAddress
      name:Brandeis University
      address:
         name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
         type:PostalAddress
      name:University of Freiburg
      address:
         name:Institute of Biology, University of Freiburg, Freiburg, Germany
         type:PostalAddress
      name:Brandeis University
      address:
         name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Jessica A. Matts
      affiliation:
            name:Brandeis University
            address:
               name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
               type:PostalAddress
            type:Organization
      name:Yuliya Sytnikova
      affiliation:
            name:Brandeis University
            address:
               name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
               type:PostalAddress
            type:Organization
      name:Gung-wei Chirn
      affiliation:
            name:Brandeis University
            address:
               name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
               type:PostalAddress
            type:Organization
      name:Gabor L. Igloi
      affiliation:
            name:University of Freiburg
            address:
               name:Institute of Biology, University of Freiburg, Freiburg, Germany
               type:PostalAddress
            type:Organization
      name:Nelson C. Lau
      affiliation:
            name:Brandeis University
            address:
               name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
      name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
      name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
      name:Institute of Biology, University of Freiburg, Freiburg, Germany
      name:Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(102)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js

CDN Services {📦}

Pbgrd

4.12s.

LINK . SPRINGER . COM {}