We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-468-5_4.

Title:
Imaging of Endothelial Progenitor Cell Subpopulations in Angiogenesis Using Quantum Dot Nanocrystals | SpringerLink
Description:
Over the last decade, research has identified a class of bone marrow-derived circulating stem cells, termed endothelial progenitor cells (EPCs), that are capable of homing to vascular lesions in the eye and contributing to pathological ocular neovascularization (NV)....
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Imaging of Endothelial Progenitor Cell Subpopulations in Angiogenesis Using Quantum Dot Nanocrystals
      pageEnd:56
      pageStart:45
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-468-5.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:NanoBiotechnology Protocols
         isbn:
            978-1-62703-468-5
            978-1-62703-467-8
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Joshua M. Barnett
            affiliation:
                  name:Vanderbilt University
                  address:
                     name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:John S. Penn
            affiliation:
                  name:Vanderbilt University
                  address:
                     name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ashwath Jayagopal
            affiliation:
                  name:Vanderbilt University
                  address:
                     name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Endothelial progenitor cells, Nanocrystals, Quantum-dots, Choroidal neovascularization, Angiogenesis, Diabetic retinopathy, Age-related macular degeneration, Acetylated low-density lipoprotein, Immunomagnetic cell isolation
      description:Over the last decade, research has identified a class of bone marrow-derived circulating stem cells, termed endothelial progenitor cells (EPCs), that are capable of homing to vascular lesions in the eye and contributing to pathological ocular neovascularization (NV). In preclinical and biological studies, EPCs are frequently identified and tracked using a intracellularly loaded fluorescent tracer, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbo cyanine perchlorate-labeled acetylated LDL (DiI-acLDL). However, this method is limited by photobleaching and insufficient quantum efficiency for long-term imaging applications. We have developed a method for conjugation of high quantum efficiency, photostable, and multispectral quantum dot nanocrystals (QD) to acLDL for long-term tracking of EPCs with improved signal-to-noise ratios. Specifically, we conjugated QD to acLDL (QD-acLDL) and used this conjugated fluorophore to label a specific CD34+ subpopulation of EPCs isolated from rat bone marrow. We then utilized this method to track CD34+ EPCs in a rat model of laser-induced choroidal neovascularization (LCNV) to evaluate its potential for tracking EPCs in ocular angiogenesis, a critical pathologic feature of several blinding conditions.
      datePublished:2013
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Book:
      name:NanoBiotechnology Protocols
      isbn:
         978-1-62703-468-5
         978-1-62703-467-8
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Vanderbilt University
      address:
         name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
         type:PostalAddress
      name:Vanderbilt University
      address:
         name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
         type:PostalAddress
      name:Vanderbilt University
      address:
         name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
         type:PostalAddress
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      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Joshua M. Barnett
      affiliation:
            name:Vanderbilt University
            address:
               name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
               type:PostalAddress
            type:Organization
      name:John S. Penn
      affiliation:
            name:Vanderbilt University
            address:
               name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
               type:PostalAddress
            type:Organization
      name:Ashwath Jayagopal
      affiliation:
            name:Vanderbilt University
            address:
               name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
      name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
      name:Vanderbilt Eye Institute, Vanderbilt University, Nashville, USA
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