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Title:
Flow Cytometric Monitoring of Residual Disease in Acute Leukemia | SpringerLink
Description:
Multiparameter flow cytometry offers the unique ability to simultaneously assess and correlate multiple cellular properties at the single cell level in a timely and efficient manner. Application of this technique to the detection of residual acute leukemia after...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Flow Cytometric Monitoring of Residual Disease in Acute Leukemia
      pageEnd:136
      pageStart:123
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-357-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Hematological Malignancies
         isbn:
            978-1-62703-357-2
            978-1-62703-356-5
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Brent L. Wood
            affiliation:
                  name:University of Washington
                  address:
                     name:Department of Laboratory Medicine, University of Washington, Seattle, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Immunophenotyping, Minimal residual disease, Flow cytometry, Acute lymphoblastic leukemia, Acute myeloid leukemia
      description:Multiparameter flow cytometry offers the unique ability to simultaneously assess and correlate multiple cellular properties at the single cell level in a timely and efficient manner. Application of this technique to the detection of residual acute leukemia after therapy has been shown to be of singular importance to monitor response to therapy and provide prognostic information. Principles and methods that allow for the sensitive detection of acute leukemia following therapy are presented. The basic protocol outlines a simple and efficient method for the labeling of white cells with monoclonal antibodies directed against cell surface antigens. A second method describes a general method for the simultaneous assessment of surface and cytoplasmic antigens using a combination of fixation followed by membrane permeabilization. An illustrative panel of validated reagents currently in use for residual disease detection for acute lymphoblastic leukemia of B or T cell lineage as well as acute myeloid leukemia is provided. Principles of data analysis that allow for the reproducible detection of small populations of abnormal hematopoietic cells in peripheral blood and bone marrow are presented.
      datePublished:2013
      isAccessibleForFree:
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      context:https://schema.org
Book:
      name:Hematological Malignancies
      isbn:
         978-1-62703-357-2
         978-1-62703-356-5
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Washington
      address:
         name:Department of Laboratory Medicine, University of Washington, Seattle, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Brent L. Wood
      affiliation:
            name:University of Washington
            address:
               name:Department of Laboratory Medicine, University of Washington, Seattle, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Laboratory Medicine, University of Washington, Seattle, USA
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